{"title":"在线粒体中加入ADP后，观察到ATP合成的爆发","authors":"K. Van Dam","doi":"10.1016/0926-6593(66)90180-9","DOIUrl":null,"url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. Evidence is presented showing that the burst in ATP synthesis, occuring on addition of ADp to rat-liver mitochondria supplemented with β-hydroxybutyrate and phosphate, is due to the oxidation of endogenous NADH and not to a “discharg” of high-energy intermediates of oxidative phosphorylation.</p></span></li><li><span>2.</span><span><p>2. The ATP burst is about 3 times the amount of NAD<sup>+</sup> formed on the addition of ADP.</p></span></li><li><span>3.</span><span><p>3. Accompanying the ATP burst and the oxidation of endogenous NADH there is an extra oxygen uptake equivalent to the oxidation of the NADH.</p></span></li><li><span>4.</span><span><p>4. The ATP burst was abolished by 15 μM dinitrophenol.</p></span></li><li><span>5.</span><span><p>5. With succinate as substrate, the ATP burst equalled the amount of NADH oxidized, indicating that reducing equivalents from NADH compete for the respiration chain with those coming from succinate.</p></span></li></ul></div>","PeriodicalId":100160,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1966-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6593(66)90180-9","citationCount":"5","resultStr":"{\"title\":\"The burst in ATP synthesis observed on addition of ADP to mitochondria\",\"authors\":\"K. Van Dam\",\"doi\":\"10.1016/0926-6593(66)90180-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p></p><ul><li><span>1.</span><span><p>1. Evidence is presented showing that the burst in ATP synthesis, occuring on addition of ADp to rat-liver mitochondria supplemented with β-hydroxybutyrate and phosphate, is due to the oxidation of endogenous NADH and not to a “discharg” of high-energy intermediates of oxidative phosphorylation.</p></span></li><li><span>2.</span><span><p>2. The ATP burst is about 3 times the amount of NAD<sup>+</sup> formed on the addition of ADP.</p></span></li><li><span>3.</span><span><p>3. Accompanying the ATP burst and the oxidation of endogenous NADH there is an extra oxygen uptake equivalent to the oxidation of the NADH.</p></span></li><li><span>4.</span><span><p>4. The ATP burst was abolished by 15 μM dinitrophenol.</p></span></li><li><span>5.</span><span><p>5. With succinate as substrate, the ATP burst equalled the amount of NADH oxidized, indicating that reducing equivalents from NADH compete for the respiration chain with those coming from succinate.</p></span></li></ul></div>\",\"PeriodicalId\":100160,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1966-11-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0926-6593(66)90180-9\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0926659366901809\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926659366901809","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The burst in ATP synthesis observed on addition of ADP to mitochondria
1.
1. Evidence is presented showing that the burst in ATP synthesis, occuring on addition of ADp to rat-liver mitochondria supplemented with β-hydroxybutyrate and phosphate, is due to the oxidation of endogenous NADH and not to a “discharg” of high-energy intermediates of oxidative phosphorylation.
2.
2. The ATP burst is about 3 times the amount of NAD+ formed on the addition of ADP.
3.
3. Accompanying the ATP burst and the oxidation of endogenous NADH there is an extra oxygen uptake equivalent to the oxidation of the NADH.
4.
4. The ATP burst was abolished by 15 μM dinitrophenol.
5.
5. With succinate as substrate, the ATP burst equalled the amount of NADH oxidized, indicating that reducing equivalents from NADH compete for the respiration chain with those coming from succinate.
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