Jinhui Liu , Lingna Zheng , Qian Li , Liuxing Feng , Bing Wang , Mingli Chen , Meng Wang , Jianhua Wang , Weiyue Feng
{"title":"同位素稀释LA-ICP-MS用于小鼠脑切片微量元素定量成像","authors":"Jinhui Liu , Lingna Zheng , Qian Li , Liuxing Feng , Bing Wang , Mingli Chen , Meng Wang , Jianhua Wang , Weiyue Feng","doi":"10.1016/j.aca.2023.341524","DOIUrl":null,"url":null,"abstract":"<div><p><span><span>Isotope dilution (ID) analysis is considered one of the most accurate </span>quantitative methods<span><span>. However, it has not been widely applied to the quantitative imaging of trace elements in biological samples using </span>laser ablation<span> inductively coupled plasma mass spectrometry (LA-ICP-MS), mainly because of difficulties in homogeneously mixing enriched isotopes (the spike) with the sample (e.g., a tissue section). In this study, we present a novel method for the quantitative imaging of trace elements (copper and zinc) in mouse brain sections using ID-LA-ICP-MS. We used an electrospray-based coating device (ECD) to evenly distribute a known amount of the spike (</span></span></span><sup>65</sup>Cu and <sup>67</sup><span>Zn) on the sections. The optimal conditions for this process involved evenly distributing the enriched isotopes on mouse brain sections mounted on indium tin oxide (ITO) glass slides using the ECD with the 10 mg g</span><sup>−1</sup> ɑ-cyano-4-hydroxycinnamic acid (CHCA) in methanol at 80 °C. The mass of the spiked isotopes and the tissue sections on the ITO slides was calculated by weighing them on an analytical balance. Quantitative images of Cu and Zn in Alzheimer's disease (AD) mouse brain sections were obtained using ID-LA-ICP-MS. These imaging results showed that Cu and Zn concentrations in various brain regions typically ranged from 10 to 25 μg g<sup>−1</sup> and 30–80 μg g<sup>−1</sup>, respectively. But it is worth noting that the hippocampus contained up to 50 μg g<sup>−1</sup> of Zn, while the cerebral cortex and hippocampus had Cu contents as high as 150 μg g<sup>−1</sup><span>. These results were validated by acid digestion and solution analysis with ICP-MS. The novel ID-LA-ICP-MS method provides an accurate and reliable means for quantitative imaging of biological tissue sections.</span></p></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":null,"pages":null},"PeriodicalIF":5.7000,"publicationDate":"2023-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Isotope dilution LA-ICP-MS for quantitative imaging of trace elements in mouse brain sections\",\"authors\":\"Jinhui Liu , Lingna Zheng , Qian Li , Liuxing Feng , Bing Wang , Mingli Chen , Meng Wang , Jianhua Wang , Weiyue Feng\",\"doi\":\"10.1016/j.aca.2023.341524\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span><span>Isotope dilution (ID) analysis is considered one of the most accurate </span>quantitative methods<span><span>. However, it has not been widely applied to the quantitative imaging of trace elements in biological samples using </span>laser ablation<span> inductively coupled plasma mass spectrometry (LA-ICP-MS), mainly because of difficulties in homogeneously mixing enriched isotopes (the spike) with the sample (e.g., a tissue section). In this study, we present a novel method for the quantitative imaging of trace elements (copper and zinc) in mouse brain sections using ID-LA-ICP-MS. We used an electrospray-based coating device (ECD) to evenly distribute a known amount of the spike (</span></span></span><sup>65</sup>Cu and <sup>67</sup><span>Zn) on the sections. The optimal conditions for this process involved evenly distributing the enriched isotopes on mouse brain sections mounted on indium tin oxide (ITO) glass slides using the ECD with the 10 mg g</span><sup>−1</sup> ɑ-cyano-4-hydroxycinnamic acid (CHCA) in methanol at 80 °C. The mass of the spiked isotopes and the tissue sections on the ITO slides was calculated by weighing them on an analytical balance. Quantitative images of Cu and Zn in Alzheimer's disease (AD) mouse brain sections were obtained using ID-LA-ICP-MS. These imaging results showed that Cu and Zn concentrations in various brain regions typically ranged from 10 to 25 μg g<sup>−1</sup> and 30–80 μg g<sup>−1</sup>, respectively. But it is worth noting that the hippocampus contained up to 50 μg g<sup>−1</sup> of Zn, while the cerebral cortex and hippocampus had Cu contents as high as 150 μg g<sup>−1</sup><span>. These results were validated by acid digestion and solution analysis with ICP-MS. The novel ID-LA-ICP-MS method provides an accurate and reliable means for quantitative imaging of biological tissue sections.</span></p></div>\",\"PeriodicalId\":240,\"journal\":{\"name\":\"Analytica Chimica Acta\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":5.7000,\"publicationDate\":\"2023-09-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analytica Chimica Acta\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0003267023007456\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytica Chimica Acta","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0003267023007456","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Isotope dilution LA-ICP-MS for quantitative imaging of trace elements in mouse brain sections
Isotope dilution (ID) analysis is considered one of the most accurate quantitative methods. However, it has not been widely applied to the quantitative imaging of trace elements in biological samples using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS), mainly because of difficulties in homogeneously mixing enriched isotopes (the spike) with the sample (e.g., a tissue section). In this study, we present a novel method for the quantitative imaging of trace elements (copper and zinc) in mouse brain sections using ID-LA-ICP-MS. We used an electrospray-based coating device (ECD) to evenly distribute a known amount of the spike (65Cu and 67Zn) on the sections. The optimal conditions for this process involved evenly distributing the enriched isotopes on mouse brain sections mounted on indium tin oxide (ITO) glass slides using the ECD with the 10 mg g−1 ɑ-cyano-4-hydroxycinnamic acid (CHCA) in methanol at 80 °C. The mass of the spiked isotopes and the tissue sections on the ITO slides was calculated by weighing them on an analytical balance. Quantitative images of Cu and Zn in Alzheimer's disease (AD) mouse brain sections were obtained using ID-LA-ICP-MS. These imaging results showed that Cu and Zn concentrations in various brain regions typically ranged from 10 to 25 μg g−1 and 30–80 μg g−1, respectively. But it is worth noting that the hippocampus contained up to 50 μg g−1 of Zn, while the cerebral cortex and hippocampus had Cu contents as high as 150 μg g−1. These results were validated by acid digestion and solution analysis with ICP-MS. The novel ID-LA-ICP-MS method provides an accurate and reliable means for quantitative imaging of biological tissue sections.
期刊介绍:
Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review.
Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.