Yanran Liu , Yajie Yang , Yining Fan , Qi Zhao , Guanyue Gao , Jinfang Zhi
{"title":"海洋污染监测微生物电化学生物传感器的可行性研究与开发","authors":"Yanran Liu , Yajie Yang , Yining Fan , Qi Zhao , Guanyue Gao , Jinfang Zhi","doi":"10.1016/j.talanta.2022.124204","DOIUrl":null,"url":null,"abstract":"<div><p><span>Electrochemical biosensor, as a real-time and rapid detection method, has rarely been explored in marine monitoring. In present work, microbial electrochemical biosensors based on two design strategies: disperse system and integrated microbial electrode, were systematically discussed and their feasibility in marine biotoxicity assessment were investigated. An isolation method was initially investigated to eliminate the potential interference and detect the biological response accurately. The influence of water salinity on the current response was eliminated by adopting the salt-tolerant bacteria </span><em>Staphylococcus aureus</em><span><span> as test microorganism and buffer solution with sufficient ionic strength. The biotoxicity of heavy </span>metal ions and pesticides were sensitively determined. Furthermore, a novel integrated microbial biosensor was designed by immobilizing </span><em>S. aureus</em><span><span> with a redox-active gel that consists of chitosan and poly (diallyl dimethyl ammonium chloride) mixture and confined potassium </span>ferricyanide<span> via electrostatic interaction. The IC</span></span><sub>50</sub> values for Cu<sup>2+</sup>, Zn<sup>2+</sup>, Cr<sub>2</sub>O<sub>7</sub><sup>2−</sup> and Ni<sup>2+</sup> were 3.01 mg/L, 1.34 mg/L, 7.64 mg/L and 9.41 mg/L, respectively. This work not only verified the feasibility of electrochemical biosensor in marine pollution monitoring, but also compared the pros and cons of two biosensor design strategies, which provide a guidance for the future development and application of marine monitoring devices based on electrochemical method.</p></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6000,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Feasibility investigation and development of microbial electrochemical biosensors for marine pollution monitoring\",\"authors\":\"Yanran Liu , Yajie Yang , Yining Fan , Qi Zhao , Guanyue Gao , Jinfang Zhi\",\"doi\":\"10.1016/j.talanta.2022.124204\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span>Electrochemical biosensor, as a real-time and rapid detection method, has rarely been explored in marine monitoring. In present work, microbial electrochemical biosensors based on two design strategies: disperse system and integrated microbial electrode, were systematically discussed and their feasibility in marine biotoxicity assessment were investigated. An isolation method was initially investigated to eliminate the potential interference and detect the biological response accurately. The influence of water salinity on the current response was eliminated by adopting the salt-tolerant bacteria </span><em>Staphylococcus aureus</em><span><span> as test microorganism and buffer solution with sufficient ionic strength. The biotoxicity of heavy </span>metal ions and pesticides were sensitively determined. Furthermore, a novel integrated microbial biosensor was designed by immobilizing </span><em>S. aureus</em><span><span> with a redox-active gel that consists of chitosan and poly (diallyl dimethyl ammonium chloride) mixture and confined potassium </span>ferricyanide<span> via electrostatic interaction. The IC</span></span><sub>50</sub> values for Cu<sup>2+</sup>, Zn<sup>2+</sup>, Cr<sub>2</sub>O<sub>7</sub><sup>2−</sup> and Ni<sup>2+</sup> were 3.01 mg/L, 1.34 mg/L, 7.64 mg/L and 9.41 mg/L, respectively. This work not only verified the feasibility of electrochemical biosensor in marine pollution monitoring, but also compared the pros and cons of two biosensor design strategies, which provide a guidance for the future development and application of marine monitoring devices based on electrochemical method.</p></div>\",\"PeriodicalId\":435,\"journal\":{\"name\":\"Talanta\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":5.6000,\"publicationDate\":\"2023-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Talanta\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0039914022010001\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Talanta","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0039914022010001","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Feasibility investigation and development of microbial electrochemical biosensors for marine pollution monitoring
Electrochemical biosensor, as a real-time and rapid detection method, has rarely been explored in marine monitoring. In present work, microbial electrochemical biosensors based on two design strategies: disperse system and integrated microbial electrode, were systematically discussed and their feasibility in marine biotoxicity assessment were investigated. An isolation method was initially investigated to eliminate the potential interference and detect the biological response accurately. The influence of water salinity on the current response was eliminated by adopting the salt-tolerant bacteria Staphylococcus aureus as test microorganism and buffer solution with sufficient ionic strength. The biotoxicity of heavy metal ions and pesticides were sensitively determined. Furthermore, a novel integrated microbial biosensor was designed by immobilizing S. aureus with a redox-active gel that consists of chitosan and poly (diallyl dimethyl ammonium chloride) mixture and confined potassium ferricyanide via electrostatic interaction. The IC50 values for Cu2+, Zn2+, Cr2O72− and Ni2+ were 3.01 mg/L, 1.34 mg/L, 7.64 mg/L and 9.41 mg/L, respectively. This work not only verified the feasibility of electrochemical biosensor in marine pollution monitoring, but also compared the pros and cons of two biosensor design strategies, which provide a guidance for the future development and application of marine monitoring devices based on electrochemical method.
期刊介绍:
Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome.
Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.