High affinity binding of perazine and desmethylperazine to human alpha 1-acid glycoprotein.

By means of gel-chromatography we have found recently a high affinity protein binding of perazine in native human sera. The affinity constant could be determined as 5.42 x 10 I/mol (Sch/ey et al., 1979), and the binding was clearly shown to be reversible. The affmity constant of perazine to human albumin is approximately more than 2 potencies less (Kinawi and Min, 1979). No evidence could be gained that formation of perazine radicals might be responsible for the high affinity binding, nor that a dissociation of the complex took place during the chromatographic run.