[大鼠卵巢产后发育的组织化学评价]。

Problemy medycyny wieku rozwojowego Pub Date : 1983-01-01
E Kaczkowska
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引用次数: 0

摘要

对卵巢发育的形态学研究较多,对其组织化学研究较少。我们之前的实验表明,20-49天是卵巢发育的关键时期。我们决定弄清楚在这一时期,什么样的生物合成起着至关重要的作用,卵巢的各个结构有什么功能。对琥珀酸、乳酸异柠檬酸、6-磷酸葡萄糖、3-和17-氢甾体脱氢酶进行了定性研究。还检测了蛋白质、核酸和脂质。总的来说,被测酶的总活性变化不大,但卵巢各成分的活性差异较大。间质腺比其他任何组织都活跃,主要是与孕激素和雄激素的生物合成有关的酶活性。鞘膜也很活跃,但不及间质腺,而且由于缺乏17- β -氢甾体脱氢酶,似乎不可能合成雄激素。
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[Histochemical evaluation of postnatal development of the rat ovary].

Many morphological studies were carried on the ovarian development and only few on histochemistry of this process. Our previous experiments showed, there was a critical period for developing ovary between 20-49 days of life. We decided to clear what kind of biosynthesis was playing essential role during this period and what functions had each structure of the ovary. The qualitative studies of following enzymes: succinic, isocitric lactate, glucoso-6-phosphate, 3-beta and 17-beta- hydroksysteroid dehydrogenases were done. Proteins, nucleic acids and lipids were also examined. In general, total activity of tested enzymes didn't varied much, but great differences between activity of ovarian elements were observed. The interstitial gland was much more active than any other structure, mainly the enzymatic activity connected with biosynthesis of gestagens and androgens was noted. Theca was also very active but less than interstitial gland, besides it seemed to have no possibility to synthetise androgens because of 17-beta- hydroksysteroid dehydrogenase absence.

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