{"title":"酵母tRNAPhe与核酸酶酶切探针插入物相互作用时的构象变化","authors":"Peter Eigil Nielsen","doi":"10.1016/0005-2787(81)90070-8","DOIUrl":null,"url":null,"abstract":"<div><p>The conformational changes of yeast-tRNA<sup>Phe</sup> induced by various intercalators have been studied using limited digestions with nucleases S<sub>1</sub> and T<sub>1</sub>. The results show an increased sensitivity to T<sub>1</sub> in the D-loop, suggesting a weakening of the D-loop-T-loop interaction. Furthermore, the results are best explained by a non-intercalative binding of the dyes, probably in the D-loop-T-loop cavity.</p></div>","PeriodicalId":100164,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis","volume":"655 1","pages":"Pages 89-95"},"PeriodicalIF":0.0000,"publicationDate":"1981-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0005-2787(81)90070-8","citationCount":"5","resultStr":"{\"title\":\"Conformational changes of yeast tRNAPhe upon interaction with intercalators probed by nuclease digestion\",\"authors\":\"Peter Eigil Nielsen\",\"doi\":\"10.1016/0005-2787(81)90070-8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The conformational changes of yeast-tRNA<sup>Phe</sup> induced by various intercalators have been studied using limited digestions with nucleases S<sub>1</sub> and T<sub>1</sub>. The results show an increased sensitivity to T<sub>1</sub> in the D-loop, suggesting a weakening of the D-loop-T-loop interaction. Furthermore, the results are best explained by a non-intercalative binding of the dyes, probably in the D-loop-T-loop cavity.</p></div>\",\"PeriodicalId\":100164,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis\",\"volume\":\"655 1\",\"pages\":\"Pages 89-95\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1981-08-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0005-2787(81)90070-8\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0005278781900708\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0005278781900708","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 5
摘要
利用核酸酶S1和T1进行有限酶切，研究了不同插入物诱导酵母trnaphe的构象变化。结果显示D-loop中对T1的敏感性增加，表明D-loop- t -loop相互作用减弱。此外，该结果最好的解释是染料的非插层结合，可能在d环- t环腔中。
Conformational changes of yeast tRNAPhe upon interaction with intercalators probed by nuclease digestion
The conformational changes of yeast-tRNAPhe induced by various intercalators have been studied using limited digestions with nucleases S1 and T1. The results show an increased sensitivity to T1 in the D-loop, suggesting a weakening of the D-loop-T-loop interaction. Furthermore, the results are best explained by a non-intercalative binding of the dyes, probably in the D-loop-T-loop cavity.
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