矮牵牛的主要叶绿素a/b结合蛋白是由多个不同核基因编码的多肽组成的。

P Dunsmuir, S M Smith, J Bedbrook
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引用次数: 0

摘要

对矮牵牛花类囊体膜集光复合物主要叶绿素a/b结合蛋白(Cab)的5个不同cDNA克隆进行了鉴定。克隆间多肽编码区核苷酸序列相似;然而,3'非翻译区是不同的。通过基因组杂交和克隆核片段的特征分析,矮牵牛核DNA表明,主要的Cab蛋白至少有16个基因。根据这些基因与不同cDNA克隆的同源性,这些基因可以被分类为至少五个小的多基因家族。两个家族各自只包含两个基因,而且这两个基因在基因组中都是紧密相连的。在一种情况下,一个基因相对于另一个基因处于倒置的方向,使得5'端相邻;在另一种情况下,这两个基因是串联排列的。其中一个家族的两个基因都在绿叶中转录。属于不同核基因家族的基因编码主要Cab蛋白的不同多肽成分。
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The major chlorophyll a/b binding protein of petunia is composed of several polypeptides encoded by a number of distinct nuclear genes.

Five distinct cDNA clones for the major chlorophyll a/b binding protein (Cab) of the petunia thylakoid membrane light-harvesting complex have been characterized. The nucleotide sequences of the polypeptide coding regions are similar between clones; however, the 3'-untranslated regions are divergent. Analysis of petunia nuclear DNA through genomic hybridizations and characterization of cloned nuclear fragments indicates there are at least sixteen genes for the major Cab protein. These genes can be classified into at least five small multigene families on the basis of their homology to the different cDNA clones. Two families contain only two genes each and in both cases the genes are closely linked in the genome. In one case, one gene is in an inverted orientation with respect to the other such that the 5' ends are adjacent; in the other case, the two genes are in a tandem array. Both genes from one of these families are transcribed in green leaves. The genes belonging to separate nuclear gene families encode different polypeptide components of the major Cab protein.

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