{"title":"白蛋白原II的六肽和五肽延伸。针对合成六肽的特异性抗体的加工","authors":"K. Weigand , Chr. Birr , M. Suter","doi":"10.1016/0005-2795(81)90116-1","DOIUrl":null,"url":null,"abstract":"<div><p>The chemically synthesized proalbumin hexapeptide was coupled to rabbit albumin with carbodiimide. Subsequently rabbits were immunized by subcutaneous injection of the conjugate. After 5 weeks the rabbits had developed antibodies against the hexapeptide, which could be detected by immunodiffusion. A sensitive enzyme-linked immunosorbent assay was developed. Using the hexapeptide and other very similar peptides as antigens, a high specificity of the antibodies against the proalbumin hexapeptide was found.</p></div>","PeriodicalId":100165,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Protein Structure","volume":"670 3","pages":"Pages 424-427"},"PeriodicalIF":0.0000,"publicationDate":"1981-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0005-2795(81)90116-1","citationCount":"16","resultStr":"{\"title\":\"The hexa- and pentapeptide extension of proalbumin II. Processing of specific antibodies against the synthetic hexapaptide\",\"authors\":\"K. Weigand , Chr. Birr , M. Suter\",\"doi\":\"10.1016/0005-2795(81)90116-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The chemically synthesized proalbumin hexapeptide was coupled to rabbit albumin with carbodiimide. Subsequently rabbits were immunized by subcutaneous injection of the conjugate. After 5 weeks the rabbits had developed antibodies against the hexapeptide, which could be detected by immunodiffusion. A sensitive enzyme-linked immunosorbent assay was developed. Using the hexapeptide and other very similar peptides as antigens, a high specificity of the antibodies against the proalbumin hexapeptide was found.</p></div>\",\"PeriodicalId\":100165,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Protein Structure\",\"volume\":\"670 3\",\"pages\":\"Pages 424-427\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1981-10-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0005-2795(81)90116-1\",\"citationCount\":\"16\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Protein Structure\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0005279581901161\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Protein Structure","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0005279581901161","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The hexa- and pentapeptide extension of proalbumin II. Processing of specific antibodies against the synthetic hexapaptide
The chemically synthesized proalbumin hexapeptide was coupled to rabbit albumin with carbodiimide. Subsequently rabbits were immunized by subcutaneous injection of the conjugate. After 5 weeks the rabbits had developed antibodies against the hexapeptide, which could be detected by immunodiffusion. A sensitive enzyme-linked immunosorbent assay was developed. Using the hexapeptide and other very similar peptides as antigens, a high specificity of the antibodies against the proalbumin hexapeptide was found.
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