氟二硝基苯对体外微管蛋白自组装的抑制作用

Young C. Lee, Richard A. Yaple, Richard Baldridge, Mark Kirsch, Richard H. Himes
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引用次数: 21

摘要

低摩尔比的1-氟-2,4-二硝基苯(FDNB)抑制了牛脑微管蛋白自组装成微管。使用[14C]FDNB的结合研究表明,一个或两个二硝基苯基团的结合足以完全抑制组装,尽管使用更高的FDNB/微管蛋白比率可以结合更多的二硝基苯基团。二硝基苯微管蛋白在组装条件下,倾向于组装成无定形的聚集体。2-巯基乙醇硫解除去二硝基苯部分。对含一个二硝基苯基的酸水解修饰微管蛋白进行纸层析和高压电泳鉴定,其残留物为s -二硝基苯基半胱氨酸。微管蛋白的β-单体在低FDNB/微管蛋白比例下优先修饰。当微管蛋白处于聚合形式时,与FDNB的反应大大减少。FDNB降低了秋水仙碱的结合活性,但不影响蛋白质中Mg(II)的含量。
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Inhibition of tubulin self-assembly in vitro by fluorodinitrobenzene

The self-assembly of bovine brain tubulin into microtubules is inhibited by low molar ratios of 1-fluoro-2,4-dinitrobezene (FDNB). Binding studies using [14C]FDNB indicate that the incorporation of between one and two dinitrophenyl groups is sufficient to inhibit assembly completely, although more dinitrophenyl groups can be incorporated using higher FDNB/tubulin ratios. Dinitrophenyltubulin, under assembly conditions, tends to assemble into amorphous aggregates. Thiolysis by 2-mercaptoethanol removes the dinitrophenyl moieties. Paper chromatography and high-voltage electrophoresis of acid-hydrolyzed modified tubulin containing one dinitrophenyl group identified the residue as S-dinitrophenylcysteine. The β-monomer of tubulin is preferentially modified at low FDNB/tubulin ratios. The reaction with FDNB is greatly reduced when tubulin is in polymerized form. FDNB reduces the colchicine binding activity but does not affect the Mg(II) content of the protein.

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