一种新的戊烷素(血清淀粉样蛋白p组分):两种四级结构的证据及其配体对自结合的影响

M. Pontet , M. D'Asnieres , D. Gache , J. Escaig , R. Engler
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引用次数: 18

摘要

用乙醇胺或磷酸乙醇胺取代琼脂糖凝胶亲和层析分离了一种新的大鼠血清蛋白。该蛋白具有血清淀粉样蛋白p组分和c反应蛋白的形态和功能特征。它包括C5环对称结构,具有非共价交联亚基,具有钙依赖的结合位点。我们称这种蛋白为大鼠血清淀粉样蛋白p组分,因为它具有人类血清淀粉样蛋白p组分的所有典型特性:它由10个亚基组成,它含有唾液酸和己糖,它形成宏观聚合物,并且它不会与肺炎球菌c -多糖沉淀。大鼠淀粉样蛋白p组分具有三个显著特性。电镜观察显示,大鼠p组分除呈五边形和叠盘状外,还具有C10循环对称结构。大鼠淀粉样蛋白p组分对特定配体如磷酸胆碱或磷酸乙醇胺具有亲和力。这些配体能够解聚自相关的大鼠p组分。鉴于这些特点,大鼠血清淀粉样蛋白p组分可作为研究淀粉样蛋白p组分与淀粉样变性关系的重要模型。
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A new pentraxin (serum amyloid P-component) in the rat: Evidence for two quaternary structures and effect of ligands on self-association

A new rat serum protein has been isolated by affinity chromatography using ethanolamine- or phosphoethanolamine-substituted agarose gels. This protein has the morphological and functional characteristics of serum amyloid P-component and C-reactive protein. It comprises C5 cyclic symmetry structure with non covalently cross-linked subunits which have calcium-dependent binding sites. We have called this protein rat serum amyloid P-component since it has all the properties typical of human serum amyloid P-component: it is made up of 10 subunits, it contains sialic acid and hexoses, it forms macroscopic polymers and it does not precipitate with pneumococcal C-polysaccharide. Rat amyloid P-component has three remarkable properties. Electron microscopy has shown that apart from pentagonal figures and stacked discs, rat P-component has a C10 cyclic symmetry structure. Rat amyloid P-component has an affinity for specific ligands, such as phosphorylcholine or phosphoethanolamine. These ligands are able to depolymerize self-associated rat P-component. With these characteristics, rat serum amyloid P-component could prove to be an important model in the study of the relations between amyloid P-component and amyloidosis.

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