A S Vandersall, R G Cameron, C J Nairn, G Yelenosky, R J Wodzinski
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引用次数: 17
摘要
黑曲霉(Aspergillus niger, syn A. ficuum) NRRL 3135粗细胞外提取物含有葡萄糖淀粉酶(exo-1,4- α - d -葡聚糖水解酶，EC 3.2.1.2)。该酶是一种糖蛋白，通过离子交换层析、层析聚焦和conconavalin a亲和层析纯化了7倍。通过SDS-PAGE和凝胶渗透色谱法测定酶的分子量为90 kDa。酶的pI为3.4。酶的最适温度为60℃，最适pH为5.0。可溶性淀粉、麦芽糖、麦芽糖三糖、麦芽糖戊二糖、麦芽糖戊二糖和异麦芽糖的Vmax分别为55.2、11.7、32.3、47.8、59.2、12.5 nKat葡萄糖/秒，相同底物的Km分别为0.09%、0.67 mM、0.76 mM、0.76 mM、0.68 mM和122.01 mM。
Identification, characterization, and partial purification of glucoamylase from Aspergillus niger (syn A. ficuum) NRRL 3135.
The crude extracellular extract of Aspergillus niger (syn A. ficuum) NRRL 3135 contains glucoamylase (exo-1,4-alpha-D-glucanohydrolase, EC 3.2.1.2). The enzyme, a glycoprotein, was purified 7-fold by ion-exchange chromatography, chromatofocusing, and conconavalin A affinity chromatography. The molecular weight of the enzyme was estimated to be 90 kDa by SDS-PAGE and gel permeation chromatography. The pI of the enzyme was 3.4. The temperature optimum of the enzyme was 60 degrees C and the pH optimum was 5.0. The Vmax values for soluble starch, maltose, maltotriose, maltotretraose, maltopentaose, and isomaltose were 55.2, 11.7, 32.3, 47.8, 59.2, 12.5 nKat glucose/sec, respectively and the Km values for the same substrates were 0.09%, 0.67 mM, 0.76 mM, 0.76 mM, 0.68 mM, and 122.01 mM, respectively.
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