膜翅目半轴蜂和青花蜂的重复DNA序列家族研究叶蜂科)。

Idengaku zasshi Pub Date : 1995-02-01 DOI:10.1266/jjg.70.7
S Sonoda, T Yamada, T Naito, F Nakasuji
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引用次数: 9

摘要

对双头半轴棘鱼和athyrii双头棘鱼的重复DNA序列进行了家族鉴定。小鲵pHMS家族和pHME家族由串联排列的序列组成,其基本重复单位分别为260 bp和330 bp。athyrii的pHAE家族是一个串联排列的序列,其基本重复单位为330bp。pHMS家族和pHME家族分别约占minomensis基因组的4.8%和0.07%。相比之下,在H. athyrii中,pHAE家族占基因组的0.04%。三个重复家族的核苷酸序列比较显示同源性很小。对6种半轴蝽的Southern blot杂交结果表明,这些重复科具有种特异性。
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Repetitive DNA sequence families in Hemitaxonus minomensis and H. athyrii (Hymenoptera; Tenthredinidae).
Families of the repetitive DNA sequences from Hemitaxonus minomensis and H. athyrii were characterized. pHMS family and pHME family in H. minomensis consist of tandemly arranged arrays whose basic repeat units are 260 bp and 330 bp, respectively. pHAE family in H. athyrii consists of a tandemly arranged array whose basic repeat unit is 330 bp. pHMS family and pHME family occupy approximately 4.8% and 0.07% of the genome of H. minomensis, respectively. By contrast, in H. athyrii, pHAE family comprise 0.04% of the genome. Nucleotide sequence comparison of these three repetitive families showed very little homology. Southern blot hybridization using six species of Hemitaxonus showed that these repetitive families are species specific.
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