改进了从冷冻视网膜中大规模纯化转导蛋白及其α和β γ亚基的方法。

Preparative biochemistry Pub Date : 1994-11-01 DOI:10.1080/10826069408010099

R E Jonas, C Yuan, T G Ebrey

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引用次数: 1

摘要

通过修改现有程序，从冷冻牛棒外段中纯化了转导蛋白异源三聚体及其α -亚基和β - γ亚基。这里描述的方法相对简单和快速。从冷冻视网膜中获得的转导蛋白异源三聚体和亚基的产量(约8毫克/100个视网膜)和纯度等于或大于以前从新鲜或冷冻视网膜中获得的。

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Improved large-scale purification of transducin, and its alpha and beta gamma subunits from frozen retinas.

The transducin heterotrimer and its alpha- and beta gamma-subunits have been purified from frozen bovine rod outer segments by modifying existing procedures. The methods described here are relatively simple and fast. The yield (ca. 8 mgs/100 retinas) and purity of the transducin heterotrimer and subunits from frozen retinas is equal to or larger than those previously obtained from fresh or frozen retinas.

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Preparative biochemistry

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