Conget I., Rasschaert J., Sener A., Leclercqmeyer V., Villanuevapenacarrillo M., Valverde I., Malaisse W.J.
{"title":"胰岛对棕榈酸酯和油酸酯的分泌、生物合成、呼吸、阳离子和代谢反应","authors":"Conget I., Rasschaert J., Sener A., Leclercqmeyer V., Villanuevapenacarrillo M., Valverde I., Malaisse W.J.","doi":"10.1006/bmmb.1994.1023","DOIUrl":null,"url":null,"abstract":"<div><p>Palmitate and oleate (0.5 to 1.0 mM) caused a time-and concentration-related augmentation of insulin release evoked by D-glucose (6.0 to 16.7 mM) in rat isolated pancreatic islets. This contrasted with an inhibitory action of the fatty acids upon L-[4-<sup>3</sup>H] phenylalanine incorporation into TCA-precipitable material, but coincided with an increased biosynthesis of proinsulin relative to that of other islet peptides. The failure of palmitate to cause an immediate increase in insulin output at a low glucose concentration (6.0 mM) coincided with an unchanged rate of O<sub>2</sub> uptake over a 10- to 15-min exposure to this fatty acid. Over prolonged incubation (90 min), however, both palmitate and oleate (1.0 mM) stimulated <sup>45</sup>Ca net uptake by islets exposed to 6.0 mM D-glucose. Like their insulinotropic effect, the time course for the oxidation of [U-<sup>14</sup>C]palmitate and [U-<sup>14</sup>C]oleate was characterized by a progressive buildup in <sup>14</sup>CO<sub>2</sub>, production rate. Moreover, palmitate and oleate decreased D-[5-<sup>3</sup>H]glucose conversion to <sup>3</sup>HOH and D-[U-<sup>14</sup>C]glucose conversion to radioactive acidic metabolites over short (30 min) but not prolonged (120 min) incubation periods. The two fatty acids also interfered with the generation of <sup>14</sup>CO<sub>2</sub>, from islets prelabeled with [U-<sup>14</sup>C]palmitate, but not L-[U-<sup>14</sup>C]glutamine. It is concluded that, at least during prolonged exposure to either palmitate or oleate, the secretory, cationic, and metabolic response to these fatty acids displays features comparable to those usually found in islets stimulated by nutrient secretagogues.</p></div>","PeriodicalId":8752,"journal":{"name":"Biochemical medicine and metabolic biology","volume":"51 2","pages":"Pages 175-184"},"PeriodicalIF":0.0000,"publicationDate":"1994-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/bmmb.1994.1023","citationCount":"24","resultStr":"{\"title\":\"Secretory, Biosynthetic, Respiratory, Cationic, and Metabolic Responses of Pancreatic Islets to Palmitate and Oleate\",\"authors\":\"Conget I., Rasschaert J., Sener A., Leclercqmeyer V., Villanuevapenacarrillo M., Valverde I., Malaisse W.J.\",\"doi\":\"10.1006/bmmb.1994.1023\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Palmitate and oleate (0.5 to 1.0 mM) caused a time-and concentration-related augmentation of insulin release evoked by D-glucose (6.0 to 16.7 mM) in rat isolated pancreatic islets. This contrasted with an inhibitory action of the fatty acids upon L-[4-<sup>3</sup>H] phenylalanine incorporation into TCA-precipitable material, but coincided with an increased biosynthesis of proinsulin relative to that of other islet peptides. The failure of palmitate to cause an immediate increase in insulin output at a low glucose concentration (6.0 mM) coincided with an unchanged rate of O<sub>2</sub> uptake over a 10- to 15-min exposure to this fatty acid. Over prolonged incubation (90 min), however, both palmitate and oleate (1.0 mM) stimulated <sup>45</sup>Ca net uptake by islets exposed to 6.0 mM D-glucose. Like their insulinotropic effect, the time course for the oxidation of [U-<sup>14</sup>C]palmitate and [U-<sup>14</sup>C]oleate was characterized by a progressive buildup in <sup>14</sup>CO<sub>2</sub>, production rate. Moreover, palmitate and oleate decreased D-[5-<sup>3</sup>H]glucose conversion to <sup>3</sup>HOH and D-[U-<sup>14</sup>C]glucose conversion to radioactive acidic metabolites over short (30 min) but not prolonged (120 min) incubation periods. The two fatty acids also interfered with the generation of <sup>14</sup>CO<sub>2</sub>, from islets prelabeled with [U-<sup>14</sup>C]palmitate, but not L-[U-<sup>14</sup>C]glutamine. 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引用次数: 24
摘要
棕榈酸酯和油酸酯(0.5 ~ 1.0 mM)引起d -葡萄糖(6.0 ~ 16.7 mM)引起的大鼠离体胰岛胰岛素释放的时间和浓度相关增加。这与脂肪酸对L-[4-3H]苯丙氨酸掺入tca沉淀物质的抑制作用形成对比,但与其他胰岛肽相比,胰岛素原的生物合成增加。棕榈酸盐在低葡萄糖浓度(6.0 mM)下不能立即引起胰岛素输出的增加,同时暴露于这种脂肪酸10- 15分钟内氧摄取速率不变。然而,经过长时间的培养(90分钟),棕榈酸酯和油酸酯(1.0 mM)均刺激暴露于6.0 mM d -葡萄糖的胰岛对45Ca的净吸收。[U-14C]棕榈酸酯和[U-14C]油酸酯的氧化过程与它们的胰岛素作用一样,其特征是14CO2的生成速率逐渐增加。此外,棕榈酸酯和油酸酯在较短(30分钟)但不延长(120分钟)的潜伏期内,降低了D-[5-3H]葡萄糖转化为3HOH和D-[U-14C]葡萄糖转化为放射性酸性代谢物。这两种脂肪酸也干扰了[U-14C]棕榈酸预标记的胰岛产生的14CO2,而不是L-[U-14C]谷氨酰胺。结论是,至少在长时间暴露于棕榈酸盐或油酸盐中,对这些脂肪酸的分泌、阳离子和代谢反应显示出与通常在营养分泌剂刺激下的胰岛中发现的特征相当。
Secretory, Biosynthetic, Respiratory, Cationic, and Metabolic Responses of Pancreatic Islets to Palmitate and Oleate
Palmitate and oleate (0.5 to 1.0 mM) caused a time-and concentration-related augmentation of insulin release evoked by D-glucose (6.0 to 16.7 mM) in rat isolated pancreatic islets. This contrasted with an inhibitory action of the fatty acids upon L-[4-3H] phenylalanine incorporation into TCA-precipitable material, but coincided with an increased biosynthesis of proinsulin relative to that of other islet peptides. The failure of palmitate to cause an immediate increase in insulin output at a low glucose concentration (6.0 mM) coincided with an unchanged rate of O2 uptake over a 10- to 15-min exposure to this fatty acid. Over prolonged incubation (90 min), however, both palmitate and oleate (1.0 mM) stimulated 45Ca net uptake by islets exposed to 6.0 mM D-glucose. Like their insulinotropic effect, the time course for the oxidation of [U-14C]palmitate and [U-14C]oleate was characterized by a progressive buildup in 14CO2, production rate. Moreover, palmitate and oleate decreased D-[5-3H]glucose conversion to 3HOH and D-[U-14C]glucose conversion to radioactive acidic metabolites over short (30 min) but not prolonged (120 min) incubation periods. The two fatty acids also interfered with the generation of 14CO2, from islets prelabeled with [U-14C]palmitate, but not L-[U-14C]glutamine. It is concluded that, at least during prolonged exposure to either palmitate or oleate, the secretory, cationic, and metabolic response to these fatty acids displays features comparable to those usually found in islets stimulated by nutrient secretagogues.