{"title":"来自大鼠腮腺质膜的高亲和力Ca2+- atp酶是一种外溶酶:Ca2+- atp酶活性的增溶和表征","authors":"Teo T.S., Thiyagarajah P., Lee M.K., Selwyn M.J.","doi":"10.1006/bmmb.1993.1076","DOIUrl":null,"url":null,"abstract":"<div><p>A high-affinity Ca<sup>2+</sup>-ATPase was solubilized from rat parotid plasma membranes and purified by concanavalin A and DEAE-cellulose chromatography. The properties of the purified high-affinity Ca<sup>2+</sup>-ATPase are very different from those of the parotid plasma membrane ATP-dependent Ca<sup>2+</sup> pump but appear to be similar to those of a rat liver cell adhesion protein which exhibits high-affinity ecto-Ca<sup>2+</sup>-ATPase activity.</p></div>","PeriodicalId":8752,"journal":{"name":"Biochemical medicine and metabolic biology","volume":"50 3","pages":"Pages 358-362"},"PeriodicalIF":0.0000,"publicationDate":"1993-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/bmmb.1993.1076","citationCount":"1","resultStr":"{\"title\":\"The High-Affinity Ca2+-ATPase from Rat Parotid Plasma Membranes Is an Ectoenzyme: Solubilization and Characterization of the Ca2+-ATPase Activity\",\"authors\":\"Teo T.S., Thiyagarajah P., Lee M.K., Selwyn M.J.\",\"doi\":\"10.1006/bmmb.1993.1076\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A high-affinity Ca<sup>2+</sup>-ATPase was solubilized from rat parotid plasma membranes and purified by concanavalin A and DEAE-cellulose chromatography. The properties of the purified high-affinity Ca<sup>2+</sup>-ATPase are very different from those of the parotid plasma membrane ATP-dependent Ca<sup>2+</sup> pump but appear to be similar to those of a rat liver cell adhesion protein which exhibits high-affinity ecto-Ca<sup>2+</sup>-ATPase activity.</p></div>\",\"PeriodicalId\":8752,\"journal\":{\"name\":\"Biochemical medicine and metabolic biology\",\"volume\":\"50 3\",\"pages\":\"Pages 358-362\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1993-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1006/bmmb.1993.1076\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochemical medicine and metabolic biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0885450583710765\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemical medicine and metabolic biology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0885450583710765","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The High-Affinity Ca2+-ATPase from Rat Parotid Plasma Membranes Is an Ectoenzyme: Solubilization and Characterization of the Ca2+-ATPase Activity
A high-affinity Ca2+-ATPase was solubilized from rat parotid plasma membranes and purified by concanavalin A and DEAE-cellulose chromatography. The properties of the purified high-affinity Ca2+-ATPase are very different from those of the parotid plasma membrane ATP-dependent Ca2+ pump but appear to be similar to those of a rat liver cell adhesion protein which exhibits high-affinity ecto-Ca2+-ATPase activity.
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