黑腹果蝇酚氧化酶原A1的遗传多态性。

Idengaku zasshi Pub Date : 1993-06-01
N Asada, K Fujimoto, M Tanaka, E Ohnishi
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引用次数: 0

摘要

从黑胃果蝇自然种群中获得了酚氧化酶原的电泳变异。在271株野生菌株的聚丙烯酰胺凝胶电泳中,发现了10种快速迁移型和2种慢迁移型。以中间迁移型居多。利用这些变异,A1基因被定位在第二染色体右臂的79.6位点。通过缺失定位,其细胞学位置定位于55A-B。在电泳图上,不同迁移率类型的杂种呈现出3条条带,表明A1是二聚体蛋白。A1基因通过幼虫、蛹和成虫阶段表达。
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Genetic polymorphism of prophenoloxidase A1 in Drosophila melanogaster.

Electrophoretic variations of prophenoloxidase were obtained from natural populations of Drosophila melanogaster. A1, one of the 2 isoforms of the prophenoloxidases, is polymorphic: 10 fast-migrating and 2 slow-migrating types were found in polyacrylamide gel electrophoresis among 271 wild strains. The majority were the intermediate-migrating type. By use of these variants, A1 gene was mapped at 79.6 on the right arm of the second chromosome. By deletion mapping, its cytological position was located at 55A-B. In the electropherograms, hybrids between the types differing in mobility exhibited 3 bands, indicating that A1 is a dimeric protein. The gene for A1 is expressed through larval, pupal and adult stages.

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