未成熟大鼠睾丸细胞n-9、n-6和n-3脂肪酸代谢的比较研究

Kjetil Retterstøl , Trine B. Haugen , Berit Woldseth , Bjørn O. Christophersen
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引用次数: 27

摘要

饲料中n-6和n-3家族的18碳和20碳脂肪酸通过一系列特定的去饱和酶和24碳中间体的链延伸代谢为22:5,n-6和22:6,n-3。这一途径受到调控,因此在组织中发现的22:6,n-3多于22:5,n-6。大鼠睾丸是一个例外,因为22:5,n-6在这个器官中大量存在。因此,与22:6,n-3相比,大鼠睾丸对于研究22:5,n-6的详细合成似乎更有意义。我们利用富含Sertoli细胞的19日龄大鼠睾丸细胞新鲜制剂,比较了1- 14c标记的n-3、n-6和n-9脂肪酸的代谢。睾丸细胞积极合成22:6,n-3和22:5,n-6,但不能从18碳和20碳前体合成22:4,n-9。在最初加入的200 mol 14c标记的C18和C20脂肪酸中,经过24小时的孵育后,发现大约20-40 mol为24碳中间体。这表明延长、去饱和和链缩短的平衡能力有利于这些细胞中24碳中间体的积累。一个例外是[1-14C]20:3,n-9,它被有效地延长到22:3,n-9,但没有延长到C24脂肪酸。我们的数据表明,n-9脂肪酸从C22−到C24的延伸率差可能是22:4,n-9合成的一个重要障碍。22:5,n-6的高效合成也可以部分解释为什么它是大鼠睾丸中主要的22碳脂肪酸。
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A comparative study of the metabolism of n-9, n-6 and n-3 fatty acids in testicular cells from immature rat

Dietary 18 and 20-carbon fatty acids of the n-6 and the n-3 families are metabolized to 22:5,n-6 and 22:6,n-3 by a sequence of specific desaturases and chain elongation via 24-carbon intermediates. This pathway is regulated so that more 22:6,n-3 than 22:5,n-6 is found in the tissues. Rat testis is an exception since 22:5,n-6 is present in large proportions in this organ. Therefore rat testis appears to be interesting for studies of the detailed synthesis of 22:5,n-6 compared with that of 22:6,n-3. By using fresh preparations of rat testicular cells from 19-day-old rats enriched in Sertoli cells, we compared the metabolism of 1-14C-labelled n-3, n-6 and n-9 fatty acids. The testicular cells actively synthesized 22:6,n-3 and 22:5,n-6, but not 22:4,n-9 from the 18 and 20-carbon precursors. Of 200 mol 14C-labelled C18 and C20 fatty acids added initially, approximately 20-40 mol were found as 24-carbon intermediates after 24 h of incubation. This indicates that the balanced capacity of elongation, desaturation and chain shortening favours the accumulation of 24-carbon intermediates in these cells. One exception was [1-14C]20:3,n-9 which was efficiently elongated to 22:3,n-9 but not to C24 fatty acids. Our data suggests that the poor elongation of n-9 fatty acids from C22 to C24 may be an important hindrance in the synthesis of 22:4,n-9. The efficient synthesis of 22:5,n-6 may also partly explain why this is the major 22-carbon fatty acid in rat testis.

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