使用十二烷基硫酸钠模拟载脂蛋白环境。使用脉冲场梯度核磁共振波谱分析肽- sds复合物的证据

Garry W Buchko , Annett Rozek , David W. Hoyt , Robert J Cushley , Michael A Kennedy
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引用次数: 30

摘要

采用脉冲场梯度核磁共振波谱法测量了与人类载脂蛋白C-I的脂质结合结构域对应的肽的平移扩散系数(Ds),残基7-24 (apoC-I(7-24))。apoC-I(7-24)的扩散系数是通过跟踪选定肽质子在不同浓度十二烷基硫酸钠(SDS)下的共振强度衰减直接确定的,SDS是一种越来越多地用于模拟载脂蛋白环境的洗涤剂。以前,多肽在SDS存在下的扩散系数是通过监测SDS扩散系数间接确定的。直接测量肽的扩散系数可以区分SDS是简单地覆盖在肽的表面以产生均匀带电的“棒”,还是肽与胶束结合。使用直接法,在SDS浓度大于5 mM(低于SDS临界胶束浓度(8.1 mM))时,apoc - 1(7-24)的扩散系数与大分子量复合物的形成一致。根据自由肽和SDS相关肽的扩散系数之比,肽- SDS复合物的分子量远大于1.4倍,如果apoc - 1(7-24)表面均匀涂覆SDS,则可以预测自由肽的分子量增加。
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The use of sodium dodecyl sulfate to model the apolipoprotein environment. Evidence for peptide–SDS complexes using pulsed-field-gradient NMR spectroscopy

Pulsed-field-gradient NMR spectroscopy was used to measure translational diffusion coefficients (Ds) for a peptide corresponding to a proposed lipid-binding domain of human apolipoprotein C-I, residues 7–24 (apoC-I(7–24)). Diffusion coefficients for apoC-I(7–24) were determined directly by following the decay of the resonance intensity of selected peptide protons at various concentrations of sodium dodecyl sulfate (SDS), a detergent increasingly being used to model the apolipoprotein environment. Previously, diffusion coefficients of peptides in the presence of SDS have been determined indirectly by monitoring the SDS diffusion coefficient. The direct measurement of the diffusion coefficient of the peptide enables one to distinguish whether SDS simply coats the peptide's surface to produce a uniformly charged `rod' or if the peptide associates with a micelle. Using the direct method, at SDS concentrations above 5 mM (which is below the SDS critical micelle concentration (8.1 mM)), apoC-I(7–24) exhibited diffusion coefficients consistent with the formation of a large-molecular-weight complex. Based on the ratio of the diffusion coefficients for free- and SDS-associated peptide, the molecular weight of the peptide–SDS complex was much larger than a factor of 1.4, the increase in molecular weight of the free peptide predicted if apoC-I(7–24) was uniformly surface coated with SDS.

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