大鼠淋巴细胞降钙素基因相关肽(CGRP)免疫反应性定位的形态学证据。

L Xing, J Guo, J Tang, Y Tang, X Wang
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引用次数: 0

摘要

降钙素基因相关肽(CGRP)广泛分布于感觉神经元和神经纤维中。本实验室最近用放射免疫法和反相高效液相色谱法发现,大鼠胸腺和肠系膜淋巴结淋巴细胞提取物中存在cgrp免疫反应性(CGRP-ir)。本研究采用免疫细胞化学方法检测CGRP-ir在大鼠淋巴细胞中的位置。取雄性Wistar大鼠胸腺和肠系膜淋巴结单细胞(200-250 g),悬浮于RPMI-1640培养基中。附着于板壁后,通过尼龙羊毛纤维柱,得到富T细胞悬浮液。细胞悬液免疫细胞化学ABC法,特异性抗血清(1:20 00稀释)为兔抗人CGRP。用Shandon Cytospin涂片于载玻片上,光镜下观察。结果显示部分淋巴细胞CGRP-ir阳性。阳性颗粒呈环状、斑块、帽状或斑点状分布于细胞表面或细胞内部。提示CGRP-ir可能存在于部分大鼠T淋巴细胞中。这项工作为神经系统和免疫系统之间的相互作用提供了新的信息。位于淋巴细胞中的CGRP的功能意义有待进一步研究。
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Morphological evidence for the location of calcitonin gene-related peptide (CGRP) immunoreactivity in rat lymphocytes.

Calcitonin gene-related peptide (CGRP) is widely distributed in sensory neurons and nerve fibers. It was shown recently in our laboratory that there was CGRP-immunoreactivity (CGRP-ir) in extract of rat lymphocyte of thymus and mesenteric lymph node by radioimmunoassay and reversed-phase HPLC. The aim of this study was to detect the CGRP-ir location in the rat lymphocyte by immunocytochemical method. Single cells isolated from thymus and mesenteric lymph node of male Wistar rat (200-250 g) were suspended in RPMI-1640 medium. After adhesion to plate wall and through nylon wool fiber columns, T cell-riched suspension was obtained. Immunocytochemical ABC method was performed in cell suspension, the specific antiserum (1:200 diluted) was rabbit anti-human CGRP. The cells were examined under light microscope after smeared on glass slide by Shandon Cytospin. The results showed that some lymphocytes were CGRP-ir positive. The positive granules were seen as ring, plaque, cap or spot distributing on the surface or inside the cell. The results indicate that CGRP-ir may be located in some rat T lymphocytes. This work provides new information about the interaction between the nervous and the immune systems. The functional significance of CGRP located in lymphocytes needs to be further studied.

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