胆固醇影响非洲猪瘟病毒感染

C. Bernardes , A. António , Maria C. Pedroso de Lima , M.L. Valdeira
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引用次数: 32

摘要

非洲猪瘟病毒(ASFV)通过受体介导的内吞作用进入细胞,在低ph下需要病毒包膜和内核体的限制膜之间的融合事件。为了研究胆固醇在ASFV感染早期的作用,我们研究了用胆固醇氧化酶处理细胞和病毒粒子去除细胞和病毒膜胆固醇的效果。以及一些胆固醇合成抑制剂对感染途径的影响。此外,我们还研究了胆固醇耗尽的Vero细胞中的病毒感染。胆固醇耗尽和胆固醇氧化酶处理的Vero细胞在结合或内化病毒的能力上没有改变,但在ASFV融合和随后的病毒复制中被阻断。我们的结果表明,ASFV感染受靶膜胆固醇的影响。
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Cholesterol affects African swine fever virus infection

African swine fever virus (ASFV) enters cells by receptor mediated endocytosis and requires a fusion event between the viral envelope and the limiting membrane of the endosome at low pH. In order to investigate the role of cholesterol in the early stages of ASFV infection, we have studied the effect of the removal of cell and viral membrane cholesterol by cholesterol oxidase treatment of cells and virions, as well as the effect of some inhibitors of cholesterol synthesis on the infectious pathway. In addition, we have investigated viral infection in cholesterol-depleted Vero cells. Both cholesterol-depleted and cholesterol oxidase-treated Vero cells were unaltered in their ability to bind or internalize the virus, but were blocked in ASFV fusion and subsequent virus replication. Our results indicate that ASFV infection is affected by cholesterol in the target membrane.

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