胆酸钠胶束的形成和胶束的增溶

Hiromi Sugioka, Yoshikiyo Moroi
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引用次数: 79

摘要

研究了298.2 K下胆酸钠(NaC)在不同pH值下的溶解度。采用不含钠离子的胆酸阴离子逐步结合模型,对胆酸胶束的聚集数(n)进行了评价,发现随着总浓度的增加,胆酸胶束的聚集数(n)增加,表明质量作用模型是有效的。在60 mM处的n值等于16。用阳离子交换膜测定了钠离子的膜电位,以确定其与胶束的低反离子结合。研究了烷基苯(苯、甲苯、乙苯、正丙苯、正丁基苯、正戊苯、正己苯)和多环芳香族化合物(萘、蒽、芘)在胆酸钠胶束水溶液中的增溶作用。在298.2 K下分光光度法测定平衡时的增溶物浓度。从平衡浓度计算了可增溶单体与空胶束之间的第一阶缔合常数K1,发现可增溶单体与空胶束之间的第一阶缔合常数K1随着可增溶单体疏水性的增加而增加。从不同平均聚集数下的吉布斯增溶能变化和增溶剂的分子结构出发,讨论了胆酸钠胶束的增溶作用,并与传统脂肪胶束的数据进行了比较。
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Micelle formation of sodium cholate and solubilization into the micelle

The micellization of sodium cholate (NaC) was studied at 298.2 K by aqueous solubility at different pH values. Using a stepwise association model of cholate anions without the sodium counterion, the aggregation number (n) of the cholate micelle was evaluated and found to increase with the total concentration, indicating that the mass action model worked quite well. The n value at 60 mM was found equal to 16. The membrane potential measurement of sodium ion with a cation exchange membrane was made in order to confirm the low counterion binding to micelle. The solubilization of alkylbenzenes (benzene, toluene, ethylbenzene, n-propylbenzene, n-butylbenzene, n-pentylbenzene, n-hexylbenzene) and polycyclic aromatic compounds (naphthalene, anthracene, pyrene) into the aqueous micellar solution of sodium cholate was carried out. Solubilizate concentrations at equilibrium were determined spectrophotometrically at 298.2 K. The first stepwise association constants (K1) between solubilizate monomer and vacant micelle were evaluated from the equilibrium concentrations and found to increase with increasing hydrophobicity of the solubilizate molecules. From the Gibbs energy change for solubilization at the different mean aggregation numbers and from molecular structure of the solubilizates, the function of sodium cholate micelle for solubilization was discussed and was compared with data from conventional aliphatic micelles.

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