胞外磷脂的掺入及其对酿酒酵母cho1/pss突变体生长和脂质代谢的影响

Jei-Oh Yon, Hidemitsu Nakamura, Akinori Ohta, Masamichi Takagi
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引用次数: 11

摘要

由于磷脂酰丝氨酸合成不足而对胆碱或乙醇胺缺乏营养的酿酒酵母cho1/pss突变体,在0.05 mM含辛酸(diC8PC)或癸酸(diC10PC)的磷脂酰胆碱(PC)存在下生长,而在含有较长酰基残基的PC存在下生长不了。它不能生长在可溶性水解产物的PC,磷胆碱或甘油磷胆碱,在相当的浓度。添加10 mM的胆碱转运抑制剂-3,或破坏编码胆碱转运蛋白的CTR基因,在胆碱存在下抑制cho1/pss突变体的生长,但在0.1 mM的diC8PC存在下则没有作用。在dic8pc支持的生长条件下,辛酸在细胞磷脂部分几乎检测不到,但在培养基中作为游离酸被回收,磷脂酰乙醇胺(PE)含量低于胆碱支持的条件。这些结果表明,具有短酰基残基的PC被cho1/pss突变体吸收并在使用时进行了改造,具有短酰基残基的PC不会抑制PE向PC的转化。目前的研究结果为酵母细胞内磷脂运动和代谢的分析提供了新的方向。
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Incorporation of extracellular phospholipids and their effect on the growth and lipid metabolism of the Saccharomyces cerevisiae cho1/pss mutant

The cho1/pss mutant of Saccharomyces cerevisiae, which is auxotrophic for choline or ethanolamine because of the deficiency in phosphatidylserine synthesis, grew in the presence of 0.05 mM phosphatidylcholine (PC) with octanoic acids (diC8PC) or decanoic acids (diC10PC), but not in the presence of PC with longer acyl residues. It did not grow in the presence of the soluble hydrolytic products of PC, phosphorylcholine or glycerophosphorylcholine, at comparable concentrations. Addition of 10 mM hemicholinium-3, a choline transport inhibitor, or disruption of the CTR gene, which encodes a choline transporter, inhibited the growth of the cho1/pss mutant in the presence of choline, but not in the presence of 0.1 mM diC8PC. Under diC8PC-supported growth conditions, octanoic acid was barely detectable in the cellular phospholipid fraction, but was recovered in the culture medium as the free acid, and the phosphatidylethanolamine (PE) content was low in comparison to the choline-supported conditions. These results suggest that PCs with short acyl residues were taken up by the cho1/pss mutant and remodeled as they were used, and that PCs with short acyl residues do not inhibit conversion of PE to PC. The current results provide a new direction in the analysis of intracellular phospholipid movement and metabolism in yeast.

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