化学核糖核酸酶切割RNA的机制和特异性。

N Beloglazova, A Vlassov, D Konevetc, V Sil'nikov, M Zenkova, R Giege, V Vlassov
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引用次数: 8

摘要

研究了1,4重氮杂环[2,2,2]辛烷与组胺和组氨酸偶联构建的化学核糖核酸酶对模型RNA底物的切割作用。与RNase A类似,化学RNase产生具有5'羟基和3'环磷酸的片段。裂解发生在催化反应中:一个RNase模拟分子可以裂解RNA中150多个磷酸二酯键。
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Mechanism and specificity of RNA cleavage by chemical ribonucleases.

Cleaving of model RNA substrates by chemical ribonucleases constructed by conjugation of 1,4 diazabicyclo[2,2,2]octane with histamine and histidine was investigated. Similarly to RNase A, the chemical RNases produce fragments with 5' hydroxy-group and 3'-cyclophosphate. The cleavage occurs as the catalytic reaction: more than 150 phosphodiester bonds in RNA can be cleaved by one molecule of RNase mimic.

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