一种评估外科手套作为病毒屏障的全手套方法。

J R Nelson, T A Roming, J K Bennett
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引用次数: 0

摘要

背景:今天,由于在卫生保健环境中遇到各种各样的感染因子,临床医生必须特别关注通过手套缺陷渗透的小尺寸病毒的可能性。目的:描述一种检测手套的方法,该方法评估整个手套并允许检测低水平的病毒渗透。采用该方法对来自4个生产厂家的10套不同的手套进行了评价。方法:采用噬菌体phiX174进行屏障性能评价。手套中充满表面活性剂溶液,放置在每mL含有10(6)个病毒的烧瓶中。烧瓶在37℃+/- 2℃下搅拌,检测180分钟。结果:100只手套中有8%的手套检测到病毒渗透。定性分析确定14%的手套允许渗透。
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A whole-glove method for the evaluation of surgical gloves as barriers to viruses.

Background: Today, because of the wide variety of infectious agents encountered in the health care environment, clinicians must be particularly concerned about the potential for small-sized virus penetration through glove defects.

Objective: To describe a method for testing gloves that evaluates the entire glove and allows for detection of low levels of virus penetration. Ten sets of 10 different gloves from 4 manufacturers were evaluated using this method.

Methods: Barrier properties were evaluated using the bacteriophage, phiX174. Gloves were filled with surfactant solution placed in flasks containing 10(6) viruses per mL. Flasks were agitated at 37 degrees C +/- 2 degrees C and assayed for 180 minutes.

Results: Virus penetration was detected in 8% of the 100 gloves tested using the quantitative assay. The qualitative assay determined that 14% of the gloves tested allowed penetration.

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