将6-甲酰基胞苷引入Myb结合序列。

Nucleosides & nucleotides Pub Date : 1999-11-01 DOI:10.1080/07328319908044640

A Kittaka, T Kuze, M Amano, H Tanaka, T Miyasaka, K Hirose, T Yoshida, A Sarai, T Yasukawa, S Ishii

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引用次数: 3

摘要

在3'-TTGAC-5'的Myb结合序列中，将单个6-甲酰基胞苷引入寡核苷酸双链(23 mers)中替代胸苷。改性双相的Tm为67℃，比原双相的Tm低6℃。23-mers与Myb蛋白的结合亲和力通过电泳迁移位移测定估计，结合几乎完全被消除。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Introduction of 6-formylcytidine into a Myb binding sequence.

Single 6-formylcytidine was introduced into a oligonucleotide duplex (23 mers) as a substitute for thymidine in the Myb binding sequence of 3'-TTGAC-5'. The modified duplex showed Tm of 67 degrees C, which was six degrees lower than the Tm of the native duplex. Binding affinity of the 23-mers to the Myb protein was estimated by electrophoretic mobility shift assays, and the binding was almost completely abolished.

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Nucleosides & nucleotides

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