微毛细管原位杂交:处理小个体样品的新方法。

IF 1.5 4区生物学 Q3 Biochemistry, Genetics and Molecular Biology Genesis Pub Date : 2000-06-01

A A Avilion, D M Bell, R Lovell-Badge

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引用次数: 0

摘要

我们已经开发出一种策略，通过RNA原位杂交来单独分析大量小组织样本。约0.4 mm x 0.5 mm的样品在矩形毛细管中处理，配有尼龙网和玻璃珠，使用标准协议。可同时测定18个样品而无损失，本底低。具体来说，我们在交配后7.5天的胚胎胚外组织绒毛膜中检测了小鼠Sox2 RNA的表达。该技术同样适用于双RNA标记，并可能用于蛋白质的抗体染色。

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Micro-capillary tube in situ hybridisation: a novel method for processing small individual samples.

We have developed a strategy to individually analyse large numbers of small tissue samples by RNA in situ hybridisation. Samples of approximately 0.4 mm x 0.5 mm are processed in rectangular capillary tubes fitted with nylon mesh and glass beads using standard protocols. Eighteen samples can be assayed simultaneously without loss, and background is low. Specifically, mouse Sox2 RNA expression is examined in the chorion of extraembryonic tissue of 7.5 days post-coitum embryos. This technique works equally well for double RNA labelling and could potentially be used for antibody staining of proteins.

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来源期刊

Genesis DEVELOPMENTAL BIOLOGY-GENETICS & HEREDITY

CiteScore

3.90

自引率

0.00%

发文量

期刊介绍： As of January 2000, Developmental Genetics was renamed and relaunched as genesis: The Journal of Genetics and Development, with a new scope and Editorial Board. The journal focuses on work that addresses the genetics of development and the fundamental mechanisms of embryological processes in animals and plants. With increased awareness of the interplay between genetics and evolutionary change, particularly during developmental processes, we encourage submission of manuscripts from all ecological niches. The expanded numbers of genomes for which sequencing is being completed will facilitate genetic and genomic examination of developmental issues, even if the model system does not fit the “classical genetic” mold. Therefore, we encourage submission of manuscripts from all species. Other areas of particular interest include: 1) the roles of epigenetics, microRNAs and environment on developmental processes; 2) genome-wide studies; 3) novel imaging techniques for the study of gene expression and cellular function; 4) comparative genetics and genomics and 5) animal models of human genetic and developmental disorders. genesis presents reviews, full research articles, short research letters, and state-of-the-art technology reports that promote an understanding of the function of genes and the roles they play in complex developmental processes.