J Catala, M Daumas, A P Chanh, B Lasserre, E Hollande
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引用次数: 6
摘要
测定兔胰管结扎术后不同时间间隔的血浆葡萄糖、胰岛素和胰高血糖素水平。观察到两个高血糖期:一个在15-90天(30天峰值15.1 +/- 1.2 mmol/l, p < 0.01),另一个在450天(11.2 +/- 0.5 mmol/l, p < 0.02)。首次高血糖发作与低胰岛素血症(41.8 +/- 8 pmol/l, r = -0.94, p < 0.01)和高胰高血糖素血症(232 +/- 21 ng/l, r = 0.95, p < 0.01)均显著相关。然而,在大量免疫染色的a细胞产生高胰高血糖素血症(390天)后,观察到晚期高血糖期(450天),不伴有低胰岛素血症,导致胰高血糖素储存增加3倍。胰岛素和胰高血糖素在180、270和450天对葡萄糖负荷的反应反映了B细胞和a细胞对葡萄糖的不敏感。由胰高血糖素主导的慢性和严重血糖紊乱的PDL兔模型模拟了继发于外分泌疾病的NIDDM综合征的关键特征。
Insulin and glucagon impairments in relation with islet cells morphological modifications following long term pancreatic duct ligation in the rabbit--a model of non-insulin-dependent diabetes.
Plasma levels of glucose, insulin and glucagon were measured at various time intervals after pancreatic duct ligation (PDL) in rabbits. Two hyperglycemic periods were observed: one between 15-90 days (peak at 30 days of 15.1 +/- 1.2 mmol/l, p < 0.01), and the other at 450 days (11.2 +/- 0.5 mmol/l, p < 0.02). The first hyperglycemic episode was significantly correlated with both hypoinsulinemia (41.8 +/- 8 pmol/l, r = -0.94, p < 0.01) and hyperglucagonemia (232 +/- 21 ng/l, r = 0.95, p < 0.01). However, the late hyperglycemic phase (450 days), which was not accompanied by hypoinsulinemia, was observed after the hyperglucagonemia (390 days) produced by abundant immunostained A-cells giving rise to a 3-fold increase in pancreatic glucagon stores. The insulin and glucagon responses to glucose loading at 180, 270 and 450 days reflected the insensitivity of B- and A-cells to glucose. The PDL rabbit model with chronic and severe glycemic disorders due to the predominant role of glucagon mimicked key features of the NIDDM syndrome secondary to exocrine disease.