{"title":"转基因玉米(Bt-maize)在猪营养中的研究:化学成分和营养评价。","authors":"T Reuter, Karen Aulrich, A Berk, G Flachowsky","doi":"10.1080/00039420214179","DOIUrl":null,"url":null,"abstract":"<p><p>The objective of the present study was to determine the composition and the nutritional value of parental and transgenic maize seeds fed to pigs. The parental maize line was genetically modified to incorporate a gene from Bacillus thuringiensis (Bt) expressing a toxin against the European corn borer (Ostrinia nubilalis). Both (parental and transgenic) maize lines were analyzed for crude nutrients, starch, sugar, non-starch polysaccharides (NSP), amino acids, fatty acids, as well as for selected minerals. Furthermore, four complete diets were mixed and were analyzed for the same nutrients and some selected ingredients. The diets contained 70% maize to attain a high effect level. To evaluate the feeding value of one variety of genetically modified maize (transgenic) compared to the feeding value of the unmodified maize (parental) line, a balance study with twelve pigs was designed. Three collecting periods were used for each maize line each with six animals. The collected faeces were analyzed for crude nutrients. All measured parameters were virtually the same (e.g. crude protein 11.59% vs. 11.06% in DM), especially the digestibility of crude protein (85.8 +/- 2.3% vs. 86.1 +/- 1.8%), the amount of nitrogen-free-extract (92.8 +/- 0.6% vs. 93.2 +/- 0.6%) and the metabolizable energy (15.7 +/- 0.2% vs. 15.8 +/- 0.2% MJ/kg DM) for both maize lines. Compared to the parental line, the chemical composition and digestibility of crude nutrients and energy content were not significantly affected by the genetic modification of maize. Therefore, from the view of a nutritional assessment, the genetically modified maize can be regarded as substantially equivalent to the parental maize line.</p>","PeriodicalId":8160,"journal":{"name":"Archiv fur Tierernahrung","volume":"56 1","pages":"23-31"},"PeriodicalIF":0.0000,"publicationDate":"2002-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/00039420214179","citationCount":"39","resultStr":"{\"title\":\"Investigations on genetically modified maize (Bt-maize) in pig nutrition: chemical composition and nutritional evaluation.\",\"authors\":\"T Reuter, Karen Aulrich, A Berk, G Flachowsky\",\"doi\":\"10.1080/00039420214179\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The objective of the present study was to determine the composition and the nutritional value of parental and transgenic maize seeds fed to pigs. 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All measured parameters were virtually the same (e.g. crude protein 11.59% vs. 11.06% in DM), especially the digestibility of crude protein (85.8 +/- 2.3% vs. 86.1 +/- 1.8%), the amount of nitrogen-free-extract (92.8 +/- 0.6% vs. 93.2 +/- 0.6%) and the metabolizable energy (15.7 +/- 0.2% vs. 15.8 +/- 0.2% MJ/kg DM) for both maize lines. Compared to the parental line, the chemical composition and digestibility of crude nutrients and energy content were not significantly affected by the genetic modification of maize. 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引用次数: 39
摘要
本研究的目的是确定饲用亲本和转基因玉米种子的成分和营养价值。对亲本玉米系进行基因改造,加入苏云金芽孢杆菌(Bt)表达抗欧洲玉米螟(Ostrinia nubilalis)毒素的基因。分析了亲本和转基因玉米系的粗营养成分、淀粉、糖、非淀粉多糖(NSP)、氨基酸、脂肪酸以及选定的矿物质。此外,将4种完全日粮混合,并对相同的营养成分和部分选定的成分进行分析。饲粮中玉米含量为70%,达到较高的效果水平。为了比较一种转基因玉米(转基因玉米)与未转基因玉米(亲本)的饲用价值,设计了12头猪的平衡试验。每个玉米品系采用3个收集期,每个收集期6头动物。收集粪便进行粗营养成分分析。2个玉米品系的粗蛋白质消化率(85.8 +/- 2.3% vs. 86.1 +/- 1.8%)、无氮提取物量(92.8 +/- 0.6% vs. 93.2 +/- 0.6%)和代谢能(15.7 +/- 0.2% vs. 15.8 +/- 0.2% MJ/kg DM)的测定参数基本相同(如粗蛋白质11.59% vs. 11.06%)。与亲本相比,转基因玉米的化学成分、粗营养物质消化率和能量含量均未受显著影响。因此,从营养评估的角度来看,转基因玉米可以被视为基本上等同于亲本玉米系。
Investigations on genetically modified maize (Bt-maize) in pig nutrition: chemical composition and nutritional evaluation.
The objective of the present study was to determine the composition and the nutritional value of parental and transgenic maize seeds fed to pigs. The parental maize line was genetically modified to incorporate a gene from Bacillus thuringiensis (Bt) expressing a toxin against the European corn borer (Ostrinia nubilalis). Both (parental and transgenic) maize lines were analyzed for crude nutrients, starch, sugar, non-starch polysaccharides (NSP), amino acids, fatty acids, as well as for selected minerals. Furthermore, four complete diets were mixed and were analyzed for the same nutrients and some selected ingredients. The diets contained 70% maize to attain a high effect level. To evaluate the feeding value of one variety of genetically modified maize (transgenic) compared to the feeding value of the unmodified maize (parental) line, a balance study with twelve pigs was designed. Three collecting periods were used for each maize line each with six animals. The collected faeces were analyzed for crude nutrients. All measured parameters were virtually the same (e.g. crude protein 11.59% vs. 11.06% in DM), especially the digestibility of crude protein (85.8 +/- 2.3% vs. 86.1 +/- 1.8%), the amount of nitrogen-free-extract (92.8 +/- 0.6% vs. 93.2 +/- 0.6%) and the metabolizable energy (15.7 +/- 0.2% vs. 15.8 +/- 0.2% MJ/kg DM) for both maize lines. Compared to the parental line, the chemical composition and digestibility of crude nutrients and energy content were not significantly affected by the genetic modification of maize. Therefore, from the view of a nutritional assessment, the genetically modified maize can be regarded as substantially equivalent to the parental maize line.