Determination of lamivudine/didanosine/nevirapine in human serum using capillary zone electrophoresis.

A combination of the anti-HIV drugs lamivudine (3TC), didanosine (ddI), and nevirapine were separated and quantitated in human serum with capillary zone electrophoresis (CZE). The effects of various factors such as run buffer concentration and pH on the separation were investigated. The optimized resolution was achieved with a run buffer containing 100 nM N,N-dimethyloctylamine in 80 mM phosphate buffer (pH 2.5). Diltiazem was chosen as the internal standard. All analytes were separated within 10 min at 30 degrees C with a voltage of +20 kV and UV detection at 210 nm.