[水稻基因组中RIRE10反转录转座子拷贝数及其LTR转录活性表征]。

Rong Wang, Guo-Fan Hong, Bin Han
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引用次数: 0

摘要

在水稻基因组4号染色体长臂上发现了一个全长ty3样反转录转座子RIRE10。两个ltr之间的内部区域在gag-pol序列上游区域有一个开放的读帧。LTR区转录本采用Northern blot杂交和RT-PCR检测。为了评估RIRE10在水稻基因组中的活性,采用点印迹法测定了其内部区域和LTR结构域的拷贝数。水稻基因组中除了65个完整的RIRE10反转录转座子旁的ltr外,还存在近900个RIRE10反转录转座子的单ltr。根据实验结果,推测RIRE10的反转录转位受两个因素的影响:LTR区的转录活性和同源重组导致的solo-LTR。
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[Characterization of the copy number of RIRE10 retrotransposon and transcriptional activity of its LTR in rice genome].

A full-length Ty3-like retrotransposon, named RIRE10, was identified on the long arm of chromosome 4 in rice genome. The internal region between two LTRs had another open reading frame in the region upstream of gag-pol sequence. The transcripts from LTR region were detected by Northern blot hybridization and RT-PCR. To assess the activity of RIRE10 in rice genome, the copy number of its internal region and long terminal repeat (LTR) domain were determined by dot blot analyses. Nearly 900 solo-LTR of the RIRE10 retrotransposon exist in rice genome, apart from those LTRs that flank 65 intact RIRE retrotransposons. Based on the experimental results, the retrotransposition of RIRE10 was speculated to be influenced by two factors: transcriptional activity of LTR region and homologous recombination resulting in solo-LTR.

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