小鼠脑内未酯化脂肪酸的体外发生及代谢

Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects Pub Date : 1964-08-05 DOI:10.1016/0926-6542(64)90006-X

C.E. Rowe

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引用次数: 15

摘要

1.1. 建立了组织提取物中未酯化脂肪酸的分离和测定方法。这是基于用重氮甲烷处理提取的脂质，然后用薄层氧化铝层析分离甲酯。已发现小鼠脑中含有相当数量的未酯化脂肪酸。主要酸为棕榈酸、硬脂酸、油酸和花生四烯酸。当小鼠脑与醋酸钠[I-14C]孵育长达6小时时，28%-66%的脂质掺入转化为未酯化的脂肪酸。在主要的酸中，只有醋酸酯被掺入到棕榈酸中。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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The occurrence and metabolism In vitro of unesterified fatty acid in mouse brain

1.
1. A method has been developed for the isolation and estimation of unesterified fatty acid in tissue extracts. This is based on treatment of extracted lipid with diazomethane followed by isolation of the methyl esters by chromatography on a thin layer of alumina.
2.
2. It has been found that mouse brain contains appreciable quantities of unesterified fatty acid.
3.
3. The principal acids were palmitic, stearic, oleic and arachidonic acids.
4.
4. When mouse brain was incubated with sodium [I-¹⁴C]acetate for periods up to 6 h, 28%–66% of the incorporation into lipid was into unesterified fatty acid.
5.
5. Of the principal acids acetate was incorporated into palmitic acid only.

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