Esteban Santiago-Calvo , Salvatore Mulé , Colvin M. Redman , Mabel R. Hokin , Lowell E. Hokin
{"title":"多磷酸肌苷的色谱分离及其在不同组织中的转化研究","authors":"Esteban Santiago-Calvo , Salvatore Mulé , Colvin M. Redman , Mabel R. Hokin , Lowell E. Hokin","doi":"10.1016/0926-6542(64)90125-8","DOIUrl":null,"url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. <sup>32</sup>P-labeled spots obtained when total lipid extracts from tissue slices are chromatographed on silicic acid-impregnated paper with phenol-ammonia as the developing solvent been characterized as di- and triphosphoinositide. This has enabled us to develop a simple, rapid, quantitative method for assaying the radioactivity incorporated into di- and triphosphoinositide in large numbers of small samples of tissue.</p></span></li><li><span>2.</span><span><p>2. Slices of salts gland, brain cortex, kidney, liver, pancreas, and heart ventricle incorporated <sup>32</sup>P into di- and triphosphoinisitide when incubated in physiological saline containing [<sup>32</sup>P]orthophosphate. With the exception of liver, the incorporation into triphosphoinositide was higher. The incorporation into diphosphoinositide generally paralled that into triphosphoinositide.</p></span></li><li><span>3.</span><span><p>3. Under conditions in which <sup>32</sup>P incorporation into phosphatidic acid and phosphatidyl inositol was stimulated by acitylcholine in salt-gland slices, there was an inhibition of incorporation into the polyphosphoinositides.</p></span></li></ul></div>","PeriodicalId":100171,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects","volume":"84 5","pages":"Pages 550-562"},"PeriodicalIF":0.0000,"publicationDate":"1964-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6542(64)90125-8","citationCount":"75","resultStr":"{\"title\":\"The chromatographic separation of polyphosphoinositides and studies on their turnover in various tissues\",\"authors\":\"Esteban Santiago-Calvo , Salvatore Mulé , Colvin M. Redman , Mabel R. Hokin , Lowell E. Hokin\",\"doi\":\"10.1016/0926-6542(64)90125-8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p></p><ul><li><span>1.</span><span><p>1. <sup>32</sup>P-labeled spots obtained when total lipid extracts from tissue slices are chromatographed on silicic acid-impregnated paper with phenol-ammonia as the developing solvent been characterized as di- and triphosphoinositide. This has enabled us to develop a simple, rapid, quantitative method for assaying the radioactivity incorporated into di- and triphosphoinositide in large numbers of small samples of tissue.</p></span></li><li><span>2.</span><span><p>2. Slices of salts gland, brain cortex, kidney, liver, pancreas, and heart ventricle incorporated <sup>32</sup>P into di- and triphosphoinisitide when incubated in physiological saline containing [<sup>32</sup>P]orthophosphate. With the exception of liver, the incorporation into triphosphoinositide was higher. The incorporation into diphosphoinositide generally paralled that into triphosphoinositide.</p></span></li><li><span>3.</span><span><p>3. Under conditions in which <sup>32</sup>P incorporation into phosphatidic acid and phosphatidyl inositol was stimulated by acitylcholine in salt-gland slices, there was an inhibition of incorporation into the polyphosphoinositides.</p></span></li></ul></div>\",\"PeriodicalId\":100171,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects\",\"volume\":\"84 5\",\"pages\":\"Pages 550-562\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1964-10-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0926-6542(64)90125-8\",\"citationCount\":\"75\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0926654264901258\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926654264901258","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The chromatographic separation of polyphosphoinositides and studies on their turnover in various tissues
1.
1. 32P-labeled spots obtained when total lipid extracts from tissue slices are chromatographed on silicic acid-impregnated paper with phenol-ammonia as the developing solvent been characterized as di- and triphosphoinositide. This has enabled us to develop a simple, rapid, quantitative method for assaying the radioactivity incorporated into di- and triphosphoinositide in large numbers of small samples of tissue.
2.
2. Slices of salts gland, brain cortex, kidney, liver, pancreas, and heart ventricle incorporated 32P into di- and triphosphoinisitide when incubated in physiological saline containing [32P]orthophosphate. With the exception of liver, the incorporation into triphosphoinositide was higher. The incorporation into diphosphoinositide generally paralled that into triphosphoinositide.
3.
3. Under conditions in which 32P incorporation into phosphatidic acid and phosphatidyl inositol was stimulated by acitylcholine in salt-gland slices, there was an inhibition of incorporation into the polyphosphoinositides.
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