重氮甲烷与合成磷脂及昆虫脂质提取物的反应

Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects Pub Date : 1964-12-02 DOI:10.1016/0926-6542(64)90025-3

H.D. Crone

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引用次数: 14

摘要

1.1. 2.2研究了重氮甲烷与合成磷脂酰乙醇胺、n -甲基磷脂酰乙醇胺、磷脂酰胆碱和昆虫脂提取物的反应，以确定该反应是否对某一类磷脂具有特异性。这三种合成磷脂基本上以相同的方式反应，尽管反应速率不同，形成磷脂酸的二甲基酯和一种类似于神经氨酸的含氮化合物。用昆虫提取物的实验结果证明了重氮甲烷与许多天然磷脂的反应。完整的乙醇胺磷脂的n -甲基化在有限的程度上发生，并且由于反应介质中存在醇而增加。所使用的技术包括硅酸柱层析、纸层析和红外光谱。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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The reaction of diazomethane with synthetic phosphatides and with insect lipid extracts

1.
1. The reaction of diazomethane with synthetic phosphatidylethanolamine, N-methylphosphatidylethanolamine, phosphatidylcholine and insect-lipid extracts was studied in order to find out if the reaction was specific to one type of phosphatide.
2.
2. The three synthetic phosphatides reacted in essentially the same manner, although with different rates of reaction, forming dimethyl esters of phosphatidic acids and a nitrogenous compound similar to neurine. The results with insect extracts gave evidence of the reaction of diazomethane with many natural phosphatides.
3.
3. N-methylation of the intact ethanolamine phosphatides occurred to a limited extent and was increased by the presnece of alcohols in the reaction medium.
4.
4. Techniques used included column chromatography on silicic acid, paper chromatography and infrared spectroscopy.

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