亚硒酸钠对端粒酶活性和端粒长度的影响。

Qiong Liu, Hong Wang, De-Cong Hu, Chao-Jian Ding, Heng Xiao, Hui-Bi Xu, Bai-Hua Shu, Shun-Qing Xu
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引用次数: 0

摘要

目的通过硒对细胞端粒酶活性和端粒长度的影响,研究硒抗衰老的生物学基础。实验中,将肝细胞L-02分为三组,分别添加终浓度为0、0.5和2.5微mol/L的亚硒酸钠。采用端粒重复扩增法和无机焦磷酸盐荧光检测法测定细胞端粒酶活性。RT-PCR半定量检测人端粒酶逆转录酶(hTERT)基因表达。用流式细胞术和荧光原位杂交技术检测端粒长度的变化。结果表明,在正常培养条件下,L-02细胞端粒酶活性和hTERT基因表达水平均较低。在添加0.5或2.5微mol/L亚硒酸钠的培养基中培养3周后,细胞生长良好。亚硒酸钠显著提高细胞端粒酶活性和hTERT基因表达水平。亚硒酸钠培养4周后,L-02细胞的端粒长度也有所延长。因此,营养剂量的亚硒酸钠可以通过增加端粒酶活性和端粒长度来延长肝细胞L-02的寿命。这一结果为解释硒的抗衰老作用提供了可能的机制。
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[Effects of sodium selenite on telomerase activity and telomere length].

To study the biological basis of selenium in resisting senescence through its effects on cellular telomerase activity and telomere length. In the experiments, the cell line of hepatocytes L-02 was divided into three groups supplemented with sodium selenite at final concentrations of 0, 0.5 and 2.5 micromol/L, respectively. Cellular telomerase activity was measured by telomeric repeat amplification protocol and enzymatic luminometric inorganic pyrophosphate detection assay. RT-PCR was used to semi-quantitatively detect human telomerase reverse transcriptase (hTERT) gene expression. The change of telomere length was assayed through flow cytometry and fluorescence in situ hybridization. Results showed that L-02 cells had low telomerase activity and hTERT gene expression level when cultured in the normal way. The cells grew well after 3-week-cultivation in the media supplemented with 0.5 or 2.5 micromol/L sodium selenite. Besides, sodium selenite significantly increased cellular telomerase activity and hTERT gene expression level. The telomere length of L-02 cells was also extended after 4-week-cultivation with sodium selenite. Thus, sodium selenite at nutritional doses could prolong the life span of hepatocytes L-02 through increasing telomerase activity and telomere length. This result provides a possible mechanism for explaining the anti-senescence function of selenium.

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Analysis of gene expression in hepatitis B virus transfected cell line induced by interferon. [Cloning and expression of tumor necrosis factor (TNFalpha) cDNA from red seabream pagrus major]. [Effects of sodium selenite on telomerase activity and telomere length]. [Cloning, eukaryotic expression and function assay of recombinant leukemia inhibitory factor gene LIF]. [The immunopotentiation of human B lymphocyte stimulator C-terminal peptide].
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