Ling Zhang, Ke Lan, Xiang-Ling Feng, Gui-Lin Qiao, Xin-Ming Shen, Yi-Min Shi, Hong Li, Kai-Tai Yao
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[Construction of N-LMP1 transgenic mice with the specific regulation region in nasopharynx].
In order to elucidate the role of EBV-LMP1 in the nasopharyngeal carcinogenesis, the expression vector was constructed with subjecting the N-LMP1 gene to double regulation of two specific regulators: EDL-2 and PLUNC-p. The N-LMP1 related transgenic mice model has been constructed successfully by pronucleus microinjection. 58 founder mice were born, 4 of which were founded to be positive by PCR and Southern blot. Immunohistochemistry assay showed that N-LMP1 protein was expressed in the nasopharynx, tongue and forestomach of transgenic mice.
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