丝裂霉素C对除草剂欧文菌冰核蛋白表达和转运的影响

Qing-Sen Chen, Xiu-Zhi Gao, Ya-Li Yan, Li-Ping Song, Guang-Chang Pang, Shu-Hua Guo
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引用次数: 0

摘要

摘要:本研究将丝裂霉素C (Mitomycin C, MMC)添加到不同培养基中,研究不同培养条件对Erwinia除草剂10025A的影响。首次证实冰核活性菌(Erwinia除草剂Erwinia除草剂10025A)中冰核活性蛋白的表达活性与其可转运方式不同。结果表明,MMC可以刺激SOS反应,诱导一些参与损伤DNA修复的酶和蛋白质的合成。不同培养基下MMC对除草剂的杀伤效果不同。它可以增加除草剂的冰核活性,形成新的小泡,这些小泡分泌到膜外。讨论了本研究对研究细胞在不良条件下的生存机制的重要性。
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[Effects of mitomycin C on the expression and transport of ice-nuclei proteins of Erwinia herbicola].

Abstract: In this paper, Mitomycin C (MMC) was added to different kinds of medium to study the effects of different cultural conditions on the Erwinia herbicola 10025A. For the first time it was confirmed that the expressed activity of the ice-nuclei active protein was different from its transportable manner from the ice nucleation active bacteria (Erwinia herbicola 10025A). The findings indicated that MMC could stimulate the SOS response,and induce the synthesis of some enzymes and proteins, which take part in repairing the damaged DNA. The effects of the MMC on the E. herbicola under different media were different. It could increase the ice nucleation activity of the E. herbicola, forming new small vesicles, which are secreted to the outside of membrane. The importance of this research for study the living mechanism of cells ander poor condition was discussed.

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