无果甙通过PPARγ/CCL2轴缓解CCI大鼠神经性疼痛。

IF 3.5 3区医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL PPAR Research Pub Date : 2021-01-22 eCollection Date: 2021-01-01 DOI:10.1155/2021/8894752

Pengfei Gao, Jiayu Wang, Zhen Su, Fayin Li, Xianlong Zhang

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引用次数: 4

摘要

神经性疼痛是一个公共健康问题。虽然许多药物被用来治疗神经性疼痛，但有效和安全的药物还不存在。在本研究中，我们测试了CCI大鼠的伤害性反应，并采用ELISA法检测了促炎细胞因子的表达。我们发现，无果素能显著降低CCI大鼠的疼痛行为，抑制脊髓中促炎因子(TNFα、IL-6和IL-1β)和趋化因子(CCL2/CCR2)的表达。然而，同时给予PPARγ拮抗剂GW9662，逆转了由无果素诱导的抗痛觉作用。结果表明，无果素通过激活PPARγ抑制炎症和趋化因子的表达，从而减轻CCI大鼠的神经性疼痛。因此，PPARγ-CCL2/CCR2通路可能是神经性疼痛的一种新的治疗选择。

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Amorfrutins Relieve Neuropathic Pain through the PPARγ/CCL2 Axis in CCI Rats.

Neuropathic pain is a public health problem. Although many pharmaceuticals are used to treat neuropathic pain, effective and safe drugs do not yet exist. In this study, we tested nociceptive responses in CCI rats, and ELISA assay was performed to examine the expression of proinflammatory cytokines. We found that amorfrutins significantly reduce the pain behaviors in CCI rats and suppress the expression of proinflammatory cytokines (TNFα, IL-6, and IL-1β) and chemokines (CCL2/CCR2) in the spinal cord. However, concurrent administration of a PPARγ antagonist, GW9662, reversed the antihyperalgesic effect induced by amorfrutins. The results indicate that amorfrutins inhibit the inflammation and chemokine expression by activating PPARγ, thus relieving neuropathic pain in CCI rats. Therefore, PPARγ-CCL2/CCR2 pathway might represent a new treatment option for neuropathic pain.

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来源期刊

PPAR Research MEDICINE, RESEARCH & EXPERIMENTAL-

CiteScore

6.20

自引率

3.40%

发文量

审稿时长

12 months

期刊介绍： PPAR Research is a peer-reviewed, Open Access journal that publishes original research and review articles on advances in basic research focusing on mechanisms involved in the activation of peroxisome proliferator-activated receptors (PPARs), as well as their role in the regulation of cellular differentiation, development, energy homeostasis and metabolic function. The journal also welcomes preclinical and clinical trials of drugs that can modulate PPAR activity, with a view to treating chronic diseases and disorders such as dyslipidemia, diabetes, adipocyte differentiation, inflammation, cancer, lung diseases, neurodegenerative disorders, and obesity.