{"title":"用于观察单分子纳米级构象变化的新荧光工具。","authors":"Erdal Toprak, Paul R Selvin","doi":"10.1146/annurev.biophys.36.040306.132700","DOIUrl":null,"url":null,"abstract":"<p><p>Single-molecule biophysics has been serving biology for more than two decades. Fluorescence microscopy is one of the most commonly used tools to identify molecules of interest and to visualize biological events. Here we describe some of the most commonly used fluorescence imaging tools to measure nanoscale movements and the rotational dynamics of biomolecules.</p>","PeriodicalId":8270,"journal":{"name":"Annual review of biophysics and biomolecular structure","volume":"36 ","pages":"349-69"},"PeriodicalIF":0.0000,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1146/annurev.biophys.36.040306.132700","citationCount":"70","resultStr":"{\"title\":\"New fluorescent tools for watching nanometer-scale conformational changes of single molecules.\",\"authors\":\"Erdal Toprak, Paul R Selvin\",\"doi\":\"10.1146/annurev.biophys.36.040306.132700\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Single-molecule biophysics has been serving biology for more than two decades. Fluorescence microscopy is one of the most commonly used tools to identify molecules of interest and to visualize biological events. Here we describe some of the most commonly used fluorescence imaging tools to measure nanoscale movements and the rotational dynamics of biomolecules.</p>\",\"PeriodicalId\":8270,\"journal\":{\"name\":\"Annual review of biophysics and biomolecular structure\",\"volume\":\"36 \",\"pages\":\"349-69\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2007-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1146/annurev.biophys.36.040306.132700\",\"citationCount\":\"70\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Annual review of biophysics and biomolecular structure\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1146/annurev.biophys.36.040306.132700\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Annual review of biophysics and biomolecular structure","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1146/annurev.biophys.36.040306.132700","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
New fluorescent tools for watching nanometer-scale conformational changes of single molecules.
Single-molecule biophysics has been serving biology for more than two decades. Fluorescence microscopy is one of the most commonly used tools to identify molecules of interest and to visualize biological events. Here we describe some of the most commonly used fluorescence imaging tools to measure nanoscale movements and the rotational dynamics of biomolecules.
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