尼古丁及其代谢物对人内皮细胞血管紧张素转换酶的影响。

Liza U Ljungberg, Karin Persson
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引用次数: 24

摘要

尼古丁已被证明可诱导内皮功能障碍,这是动脉粥样硬化的早期标志。尼古丁在肝脏中进行广泛的代谢,形成许多主要和次要的代谢物。关于尼古丁代谢物对心血管系统的影响的数据非常有限。本研究探讨了尼古丁及其代谢物可替宁、可替宁- n-氧化物、尼古丁-1′- n-氧化物、诺可替宁、反式-3′-羟基可替宁对人内皮细胞血管紧张素转换酶(ACE)的影响。用尼古丁或尼古丁代谢物刺激培养的人脐带静脉内皮细胞(HUVECs),其浓度与吸烟时血浆中观察到的浓度相似。使用商用试剂盒分析ACE活性和表达。结果表明,烟碱和烟碱代谢物均能增加ACE的活性和表达。然而,在对药物的反应中观察到明显的个体差异。这种变异与ACE插入/删除多态性无关。烟草含有大量的化学成分,吸烟者动脉粥样硬化的潜在原因可能是多因素的。这项研究的结果可以解释吸烟对血管系统产生负面影响的一种细胞机制。
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Effect of nicotine and nicotine metabolites on angiotensin-converting enzyme in human endothelial cells.

Nicotine has been shown to induce endothelial dysfunction, which is an early marker of atherosclerosis. Nicotine undergoes extensive metabolism in the liver, forming a number of major and minor metabolites. There are very limited data on the effect of nicotine metabolites on the cardiovascular system. This study investigates the effects of nicotine and the nicotine metabolites, cotinine, cotinine-N-oxide, nicotine-1'-N-oxide, norcotinine, trans-3'-hydroxycotinine, on angiotensin-converting enzyme (ACE) in human endothelial cells. Cultured endothelial cells obtained from human umbilical cord vein (HUVECs) were stimulated with nicotine or nicotine metabolites in concentrations similar to those observed in plasma during smoking. ACE activity and expression were analyzed using commercial kits. The results showed that nicotine and nicotine metabolites can increase both activity and expression of ACE. However, a marked individual variation in the response to the drugs was observed. This variation was not associated with the ACE insertion/deletion polymorphism. Tobacco contains numerous chemical compounds, and the underlying cause for development of atherosclerosis in smokers is probably multifactorial. The results from this study could explain one cellular mechanism by which smoking exerts negative effect on the vascular system.

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