内皮外和构成性COX-2表达参与大鼠主动脉血管紧张素II的收缩作用

M. C. Castillo-Hernández, M. A. Martinez-Godinez, G. Guevara-Balcazar, A. Miliar-Garcia, J. Mancilla, R. M. Lopez-Mayorga, E. F. Castillo-Henkel, C. Castillo-Henkel
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引用次数: 2

摘要

1利用年轻Wistar大鼠无内皮的胸腹主动脉环,研究了环氧化酶-2 (COX-2)内皮外和组成型异构体在血管紧张素II (Ang II)收缩作用中的作用。2 Ang II在两个主动脉段引起类似的收缩,并且用NS398(一种选择性COX-2抑制剂)预处理可以抑制这种作用,但SC-560(一种选择性环氧化酶-1 (COX-1)抑制剂)不能抑制这种作用。3 COX-2 mRNA在基底条件下均有表达。此外,Ang II增加了腹部而不是胸部的COX-2 mRNA表达,而环己亚胺(一种蛋白质合成抑制剂)不影响两个节段对Ang II的收缩反应;这表明该效应与从头合成COX-2无关。综上所述,在主动脉平滑肌细胞中发现的COX-2的基础量足以解释与Ang II收缩作用相关的前列腺素的产生。这些前列腺素的产生,来源于组成型COX-2,独立于内皮血管系统发生。
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Extraendothelial and constitutive COX-2 expression is involved in the contractile effect of angiotensin II in the rat aorta

1 The role of the extraendothelial and constitutive isoforms of cyclo-oxygenase-2 (COX-2) in the contractile effect of angiotensin II (Ang II) was investigated using thoracic and abdominal aortic rings without endothelium from young Wistar rats.

2 Ang II elicited similar contractions in both aortic segments, and the effect was inhibited by pretreatment with NS398 (a selective COX-2 inhibitor) but not SC-560 [selective cyclo-oxygenase-1 (COX-1) inhibitor].

3 COX-2 mRNA was expressed under basal conditions in both aortic segments. Additionally, Ang II increased COX-2 mRNA expression in the abdominal but not the thoracic segment, while cycloheximide (a protein synthesis inhibitor) did not affect the contractile response to Ang II in either of the two segments; this suggests that the effect is not associated with de novo COX-2 synthesis.

4 In conclusion, the basal amount of COX-2 found in aortic smooth muscle cells is sufficient to explain the production of the prostanoids related to the contractile effect of Ang II. The production of these prostanoids, which are derived from constitutive COX-2, occurs independently of the endothelium vascular system.

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Issue Information Autonomic and Autacoid Pharmacology: Goodbye and thank you Attenuation of the anti-contractile effect of cooling in the rat aorta by perivascular adipose tissue Retraction: Dopamine receptor immunohistochemistry in the rat choroid plexus. Issue Information
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