[2'-醛寡核苷酸:合成及其在dna识别蛋白亲和修饰中的应用]。

Bioorganicheskaia khimiia Pub Date : 2010-05-01
E A Khomiakova, E V Kazanova, E M Zubin, E A Kubareva, N V Molochkov, E M Riazanova, T S Oretskaia
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引用次数: 0

摘要

以2′-o -[2-(2,3-二羟丙基)氨基-2-氧乙基]尿苷3′-磷酰胺为原料制备了具有1,2-二醇基团的寡核苷酸。研究了修饰DNA双链的热稳定性及其与NF-kappaB转录因子p50亚基和(胞嘧啶-5)-DNA甲基转移酶SsoII形成复合物的能力。寡核苷酸的1,2 -二醇基团的高碘酸氧化产生活性的2'-醛衍生物。研究了它们用于dna识别蛋白亲和修饰的机会。
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[2'-aldehyde oligonucleotides: synthesis and use for affinity modification of DNA-recognizing proteins].

Oligonucleotides with 1,2-diol grouping were prepared from 2'-O-[2-(2,3-dihydroxypropyl)amino-2-oxo-ethyl]uridine 3'-phosphoramidite. The thermal stability of modified DNA duplexes and their ability to form complexes with the p50 subunit of the NF-kappaB transcription factor and (cytosine-5)-DNA methyltransferase SsoII were studied. The periodate oxidation of the l,2-diol grouping of the oligonucleotides resulted in reactive 2'-aldehyde derivatives. The opportunity of their use for the affinity modification of DNA-recognizing proteins was studied.

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