{"title":"Al2O3上游离和固定化念珠菌脂肪酶的生化特性比较研究。","authors":"Yeşim Yeşiloğlu, Latifşah Şit","doi":"10.3109/10731199.2010.533125","DOIUrl":null,"url":null,"abstract":"<p><p>Lipase from Candida rugosa (CRL) was immobilized by physical adsorption onto Al(2)O(3). The immobilization yield was more than 95% during 30 min. The properties of free and immobilized enzyme were also searched and compared. The optimum pH was shifted from 7 to 8.5 by immobilization of the enzyme. The maximum activity of the free and the immobilized enzymes occured at 35 and 40°C, respectively.</p>","PeriodicalId":8413,"journal":{"name":"Artificial cells, blood substitutes, and immobilization biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2011-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199.2010.533125","citationCount":"6","resultStr":"{\"title\":\"Biochemical properties of free and immobilized Candida rugosa lipase onto Al2O3: a comparative study.\",\"authors\":\"Yeşim Yeşiloğlu, Latifşah Şit\",\"doi\":\"10.3109/10731199.2010.533125\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Lipase from Candida rugosa (CRL) was immobilized by physical adsorption onto Al(2)O(3). The immobilization yield was more than 95% during 30 min. The properties of free and immobilized enzyme were also searched and compared. The optimum pH was shifted from 7 to 8.5 by immobilization of the enzyme. The maximum activity of the free and the immobilized enzymes occured at 35 and 40°C, respectively.</p>\",\"PeriodicalId\":8413,\"journal\":{\"name\":\"Artificial cells, blood substitutes, and immobilization biotechnology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2011-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.3109/10731199.2010.533125\",\"citationCount\":\"6\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Artificial cells, blood substitutes, and immobilization biotechnology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3109/10731199.2010.533125\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2010/11/30 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Artificial cells, blood substitutes, and immobilization biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3109/10731199.2010.533125","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2010/11/30 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
Biochemical properties of free and immobilized Candida rugosa lipase onto Al2O3: a comparative study.
Lipase from Candida rugosa (CRL) was immobilized by physical adsorption onto Al(2)O(3). The immobilization yield was more than 95% during 30 min. The properties of free and immobilized enzyme were also searched and compared. The optimum pH was shifted from 7 to 8.5 by immobilization of the enzyme. The maximum activity of the free and the immobilized enzymes occured at 35 and 40°C, respectively.
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