Lijing Sun, Yongdong Huang, Yan Zhang, Qingqiang Meng, Jian Luo, Bei Fan, Guanghui Ma, Zhiguo Su
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A simple and rapid procedure for purification of haptoglobin from human plasma fraction IV.
Human plasma fraction IV is an intermediate precipitate during the production of human serum albumin using cold ethanol method. Haptoglobin locates in this fraction can be purified for various applications. A new process integration of polyethylene glycol (PEG) precipitation and ion-exchange chromatography (IEC) was developed for purification of haptoglobin, which could effectively purify the haptoglobin from 16.6% to 95%. The recovery of the new process was 58.2% in comparison to 30.3% of the conventional affinity chromatography. Furthermore, 175 mg haptoglobin production in a scaled-up process showed the method to be simple, fast, and low-cost.
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