Pleurotus pulmonarius 和 Pleurotus ostreatus 真菌代谢产物对苯诱导的 Wister 大鼠白血病的抗白血病和免疫调节作用。

The Korean Journal of Hematology Pub Date : 2012-03-01 Epub Date: 2012-03-28 DOI:10.5045/kjh.2012.47.1.67
Akanni E Olufemi, Alli O A Terry, Oloke J Kola
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摘要

背景:在传统化疗中使用天然生物活性化合物是癌症治疗的一个新方向,近来受到越来越多的研究关注。从一些真菌(食用菌)中提取的生物活性多糖和多糖蛋白质复合物已被确定为有效、无毒的抗肿瘤药物来源。我们在一种新型酵母提取物培养基上培养了一些杏鲍菇(Pleurotus pulmonarius 和 P. ostreatus,分别为尼日利亚本地菌株和外来菌株),并补充了塞内加尔杏鲍菇的乙醇提取物,研究了其代谢物的抗白血病潜力:连续 3 周每 2 天静脉注射(0.2 mL)苯溶液,成功诱导 Wister 大鼠患白血病。在诱导白血病之前、期间和之后,口服由浸没发酵产生的真菌代谢物水溶液(20 mg/mL)(0.2 mL)。通过比较基线和白血病诱导后的血液学参数来评估白血病负担。代谢物的免疫调节潜力通过吞噬细胞检测法(碳清除法)进行评估。通过白细胞总数来评估代谢物促进白细胞生成的能力:结果:诱导白血病会导致明显的贫血指数和白细胞增多(PC结论:代谢物具有很强的抗白血病能力:这些代谢物具有很强的抗白血病潜力,可抑制白血病,并在不同实验组动物口服后显示出免疫治疗活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Anti-leukemic and immunomodulatory effects of fungal metabolites of Pleurotus pulmonarius and Pleurotus ostreatus on benzene-induced leukemia in Wister rats.

Background: The use of natural bioactive compounds in conventional chemotherapy is a new direction in cancer treatment that is gaining more research attention recently. Bioactive polysaccharides and polysaccharide-protein complexes from some fungi (edible mushrooms) have been identified as sources of effective and non-toxic antineoplastic agents. Selected oyster mushrooms (Pleurotus pulmonarius and P. ostreatus being local [Nigeria] and exotic strains, respectively) were cultured on a novel medium of yeast extract supplemented with an ethanolic extract of Annona senegalensis, and the antileukemic potential of their metabolites was studied.

Methods: Leukemia was successfully induced in Wister rats by intravenous injection (0.2 mL) of a benzene solution every 2 days for 3 consecutive weeks. The aqueous solution of fungal metabolites (20 mg/mL) produced by submerged fermentation was orally administered (0.2 mL) before, during, and after leukemia induction. Leukemia burden was assessed by comparing the hematological parameters at baseline and after leukemia induction. The immunomodulatory potential of the metabolites was assessed by using a phagocytic assay (carbon clearance method). The ability to enhance leukopoiesis was assessed by using the total leukocyte count.

Results: Leukemia induction resulted in significant anemia indices and leukocytosis (P<0.05) in the experimental rats. Both metabolites equally enhanced leukopoiesis and demonstrated phagocytic actions; P. ostreatus activity was significantly higher than that of P. pulmonarius (P<0.05).

Conclusion: The metabolites exhibited profound antileukemic potential by suppressing leukemia and demonstrating immunotherapeutic activities on animals after oral administration in various experimental groups.

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