Song Bai, Zheng-Gui Huang, Li Chen, Jiang-Tao Wang, Bo-Ping Ding
{"title":"非洛地平联合葛根素对肾血管性高血压大鼠ACE2-Ang (1-7) -Mas轴的影响","authors":"Song Bai, Zheng-Gui Huang, Li Chen, Jiang-Tao Wang, Bo-Ping Ding","doi":"10.1016/j.regpep.2013.03.005","DOIUrl":null,"url":null,"abstract":"<div><p><span>This study aimed to investigate the effect of combination of felodipine</span> <!-->+<!--> <span>puerarin on ACE2–Ang (1–7)–Mas axis, and to explore the protective effect of the combination against kidney in renovascular hypertensive rats. Goldblatt rats were randomly divided into 5 groups as follows: 4 groups which were treated with felodipine (Felo), puerarin (Pue), Felo</span> <!-->+<!--> <!-->Pue, and Felo<!--> <!-->+<!--> <span>captopril<span> (Cap), respectively, and a control group of animals that were administrated with distilled water. Contents of Ang II and Ang (1–7) in renal tissues were determined by ELISA kit. The mRNA expression of ACE2/Mas and ACE/AT</span></span><sub>1</sub> in kidneys was analyzed by RT-PCR. After 8<!--> <!-->weeks of treatment, compared with Goldblatt group, Felo<!--> <!-->+<!--> <span><span>Pue reduced SBP, </span>DBP and HR (p</span> <!--><<!--> <!-->0.01 or p<!--> <!--><<!--> <span>0.05), ameliorated renal interstitial fibrosis, decreased the level of Ang II and increased that of Ang (1–7), upregulated mRNA expression of ACE2 and Mas, decreased that of ACE and AT</span><sub>1</sub><span>, and downregulated protein expression of TGF-β</span><sub>1</sub> in kidneys (p<!--> <!--><<!--> <!-->0.01). Compared with Felo group, Felo<!--> <!-->+<!--> <!-->Pue decreased DBP and HR more markedly, attenuated fibrosis, decreased Ang II levels and increased those of Ang (1–7), upregulated mRNA expression of ACE2 in bilateral kidneys and that of Mas in ischemic kidney, downregulated that of ACE in bilateral kidneys and that of AT<sub>1</sub> in ischemic kidney, and decreased expression of TGF-β<sub>1</sub> protein significantly. In a word, a combination of Felo<!--> <!-->+<!--> <!-->Pue has a more efficient therapeutic effect on DBP and HR, and contributes to a better protection against renal interstitial fibrosis.</p></div>","PeriodicalId":20853,"journal":{"name":"Regulatory Peptides","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2013-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.regpep.2013.03.005","citationCount":"16","resultStr":"{\"title\":\"Effects of felodipine combined with puerarin on ACE2–Ang (1–7)–Mas axis in renovascular hypertensive rat\",\"authors\":\"Song Bai, Zheng-Gui Huang, Li Chen, Jiang-Tao Wang, Bo-Ping Ding\",\"doi\":\"10.1016/j.regpep.2013.03.005\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span>This study aimed to investigate the effect of combination of felodipine</span> <!-->+<!--> <span>puerarin on ACE2–Ang (1–7)–Mas axis, and to explore the protective effect of the combination against kidney in renovascular hypertensive rats. Goldblatt rats were randomly divided into 5 groups as follows: 4 groups which were treated with felodipine (Felo), puerarin (Pue), Felo</span> <!-->+<!--> <!-->Pue, and Felo<!--> <!-->+<!--> <span>captopril<span> (Cap), respectively, and a control group of animals that were administrated with distilled water. Contents of Ang II and Ang (1–7) in renal tissues were determined by ELISA kit. The mRNA expression of ACE2/Mas and ACE/AT</span></span><sub>1</sub> in kidneys was analyzed by RT-PCR. After 8<!--> <!-->weeks of treatment, compared with Goldblatt group, Felo<!--> <!-->+<!--> <span><span>Pue reduced SBP, </span>DBP and HR (p</span> <!--><<!--> <!-->0.01 or p<!--> <!--><<!--> <span>0.05), ameliorated renal interstitial fibrosis, decreased the level of Ang II and increased that of Ang (1–7), upregulated mRNA expression of ACE2 and Mas, decreased that of ACE and AT</span><sub>1</sub><span>, and downregulated protein expression of TGF-β</span><sub>1</sub> in kidneys (p<!--> <!--><<!--> <!-->0.01). Compared with Felo group, Felo<!--> <!-->+<!--> <!-->Pue decreased DBP and HR more markedly, attenuated fibrosis, decreased Ang II levels and increased those of Ang (1–7), upregulated mRNA expression of ACE2 in bilateral kidneys and that of Mas in ischemic kidney, downregulated that of ACE in bilateral kidneys and that of AT<sub>1</sub> in ischemic kidney, and decreased expression of TGF-β<sub>1</sub> protein significantly. In a word, a combination of Felo<!--> <!-->+<!--> <!-->Pue has a more efficient therapeutic effect on DBP and HR, and contributes to a better protection against renal interstitial fibrosis.</p></div>\",\"PeriodicalId\":20853,\"journal\":{\"name\":\"Regulatory Peptides\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2013-06-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.regpep.2013.03.005\",\"citationCount\":\"16\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Regulatory Peptides\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0167011513000347\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Regulatory Peptides","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0167011513000347","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Effects of felodipine combined with puerarin on ACE2–Ang (1–7)–Mas axis in renovascular hypertensive rat
This study aimed to investigate the effect of combination of felodipine + puerarin on ACE2–Ang (1–7)–Mas axis, and to explore the protective effect of the combination against kidney in renovascular hypertensive rats. Goldblatt rats were randomly divided into 5 groups as follows: 4 groups which were treated with felodipine (Felo), puerarin (Pue), Felo + Pue, and Felo + captopril (Cap), respectively, and a control group of animals that were administrated with distilled water. Contents of Ang II and Ang (1–7) in renal tissues were determined by ELISA kit. The mRNA expression of ACE2/Mas and ACE/AT1 in kidneys was analyzed by RT-PCR. After 8 weeks of treatment, compared with Goldblatt group, Felo + Pue reduced SBP, DBP and HR (p < 0.01 or p < 0.05), ameliorated renal interstitial fibrosis, decreased the level of Ang II and increased that of Ang (1–7), upregulated mRNA expression of ACE2 and Mas, decreased that of ACE and AT1, and downregulated protein expression of TGF-β1 in kidneys (p < 0.01). Compared with Felo group, Felo + Pue decreased DBP and HR more markedly, attenuated fibrosis, decreased Ang II levels and increased those of Ang (1–7), upregulated mRNA expression of ACE2 in bilateral kidneys and that of Mas in ischemic kidney, downregulated that of ACE in bilateral kidneys and that of AT1 in ischemic kidney, and decreased expression of TGF-β1 protein significantly. In a word, a combination of Felo + Pue has a more efficient therapeutic effect on DBP and HR, and contributes to a better protection against renal interstitial fibrosis.
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Regulatory Peptides provides a medium for the rapid publication of interdisciplinary studies on the physiology and pathology of peptides of the gut, endocrine and nervous systems which regulate cell or tissue function. Articles emphasizing these objectives may be based on either fundamental or clinical observations obtained through the disciplines of morphology, cytochemistry, biochemistry, physiology, pathology, pharmacology or psychology.
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