[药物“肝素”的荧光成分及其与苯丙氨酸、酪氨酸和色氨酸络合的研究]。

S S Astakhov, V M Iunusov, M V Sultanbaev, G Kh Akhmadeeva, M S Iunusov
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引用次数: 0

摘要

采用光谱发光和分光光度法研究了市售药物肝素(Hep)的氨基酸(AA)和蛋白质组分的组成及其与色氨酸、酪氨酸和苯丙氨酸的相互作用。肝素类药物中发现了芳香族氨基酸苯丙氨酸、酪氨酸和弹性蛋白的杂质。研究了Trp、Tyr和Phe在肝素作用下的猝灭荧光(FL),并测定了其与氨基酸配合物稳定性的Stern-Volmer常数(K)。配合物AA…Hep增加的序列为K (Trp…Hep) = 19 +/- 2 M(-1) < K (Tyr…Hep) = 39 +/- 3 M(-1) < K (Phe…Hep) = 710 +/- 70 M(-1),这可能决定了肝素中以Phe杂质为主而色氨酸缺失的原因。据推测,动物弹性蛋白与人类弹性蛋白不同,可引起过敏反应直至过敏性休克。
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[Investigation of fluorescent components of drug "heparin" and its complexing with phenylalanine, tyrosine and tryptophan].

Using spectral-luminescent and spectrophotometric methods a composition of aminoacidic (AA) and protein components of commercial drug of heparin (Hep) as well as its interaction with Trp, Tyr and Phe was studied. The impurities of aromatic aminoacids Phe, Tyr and elastin protein was revealed in drug of heparin. The quenching of fluorescence (FL) of Trp, Tyr and Phe with heparin was studied and the Stern-Volmer Constants (K) showing the stability of its complexes with aminoacids were determined. The stability of complexes AA...Hep increases in the series K (Trp...Hep) = 19 +/- 2 M(-1) < K (Tyr...Hep) = 39 +/- 3 M(-1) < K (Phe...Hep) = 710 +/- 70 M(-1), that, probably, determines dominating contribution of Phe impurity in heparin and the absence of that of tryptophan. It was assumed, that animal elastin, which is different from human one can provoke allergic reactions up to anaphylactic shock.

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