通过调节酵母生长能量优化生物乙醇生产。

Systems and Synthetic Biology Pub Date : 2012-12-01 Epub Date: 2012-11-10 DOI:10.1007/s11693-012-9099-6
Emad Y Moawad
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引用次数: 19

摘要

本研究旨在通过调节酵母生长能(YGE)来优化发酵生产生物乙醇,为以酵母(酿酒酵母)为接种体从食物垃圾渗滤液(FWL)中生产乙醇提供可预测和可控的机理。还原糖浓度(RSC)的范围很广,通常从低(每升35克)到很高(每升100克),除了有FWL污染和酵母细胞死亡的风险外,还造成了成本的增加。提出了一个数学模型来描述酵母生长能(YGE)由于RSC剂量以及预测乙醇产量的每一个剂量,以确定最佳的一个。该模型的仿真结果表明,发酵过程中YGE、能量摄入(EI)和它们产生的乙醇能量(PEE)根据能量守恒定律始终处于平衡状态。为了在连续工艺和大规模生产中获得更好的发酵率;YGE应小于EI的一半,大于EI的四分之一(即[公式:见文本]),这使剩余能量小于YGE,以避免渗透压力或细胞老化的风险,从而使所有酵母细胞尽可能长时间地存活,从而最大限度地提高乙醇产量并降低生产成本。
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Optimizing bioethanol production by regulating yeast growth energy.

The goal of this work is to optimize production of bio-ethanol by fermentation through regulating yeast growth energy (YGE), and provide the mechanism of ethanol production from food-waste leachate (FWL) using yeast (S. cerevisiae) as inoculums to be predictable and controllable. The wide range of reduced sugar concentration (RSC) which is commonly administered from low (35 g per liter) to very high (100 g per liter) is responsible for costs increasing besides risks of FWL contamination and death of yeast cells. A mathematical model is presented to describe yeast growth energy (YGE) due to RSC doses along with predicting the amounts of ethanol yield by each dose to identify the optimum one. Simulations of the presented model showed that YGE, energy intake (EI), and their produced ethanol energy (PEE) are always balanced during fermentation process according to the law of conservation of energy. For a better fermentation rate in a continuous process and a large-scale production; YGE should be less than half of EI and more than its quarter (i.e. [Formula: see text]) which keeps the residual energy less than YGE to avoid risks of osmotic stresses or aging of cells allowing the survival of all yeast cells as long as possible to maximize ethanol production and decrease productivity costs.

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