[血清分型法测定百日咳杆菌Fim2和Fim3抗原的重复性]。

Monika Zawadka, Bozena Moskala, Iwona Letowska, Ewa Mosiej, Katarzyna Krysztopa-Grzybowska, Anna Lutyńska
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引用次数: 0

摘要

简介:血清分型是一种常用的方法,用于描述百日咳博德特拉菌株中菌毛2和菌毛3的存在,用于流行病学目的和百日咳全细胞疫苗菌株组成的最佳选择。推荐使用单克隆抗Fim2和Fim3血清进行微孔板血清分型,而不是多克隆。百日咳菌株表达的菌膜的可靠评价影响疫苗驱动菌株进化的解释。方法:为了评估测试条件对血清分型可重复性的影响,比较了三个不同认可实验室基于单克隆抗血清微孔板凝集标准化方案的血清分型结果。本研究比较了3株百日咳疫苗株在来自不同生物滤池的种子批次系统内的分离株。结果:三个实验室对血清分型结果缺乏完全一致,可能与所用培养基、主观读数、测试条件和试剂特异性的差异有关。结论:血清分型结果应谨慎解释,在验证研究后应准确推荐使用的培养基类型和培养条件。不一致的结果应采用另一种技术进行确认。ELISA或参比实验室检测。
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[Reproducibility of Fim2 and Fim3 antigens determination in Bordetella pertussis by serotyping method].

Introduction: Serotyping is a commonly used method to characterize the presence of Fimbriae 2 and 3 in Bordetella pertussis strains for epidemiological purposes and optimal choice of strain composition of the pertussis whole-cell vaccine. Monoclonal antisera against Fim2 and Fim3 are recommended to be used for microplate serotyping instead ofpolyclonal. Reliable evaluation offimbriae expressed by B. pertussis strains influence interpretation of vaccine-driven strain evolution.

Methods: To evaluate the impact of tests conditions on the reproducibility of serotyping, results of serotyping based on a standardized protocol for microplate agglutination with monoclonal antisera performed in three different accredited laboratories were compared. For the study isolates of three vaccine strains of B. pertussis deposited within seed lot system originating from different liofilization lots were compared.

Results: Lack of the complete agreement on serotyping results among three labs might relates to the differences of media used, subjective reading, test conditions, and specificity of the reagents.

Conclusions: Serotyping results should be interpreted with caution and the type of media and culture conditions used should be precisely recommended after validation studies. Inconsistent results should be confirmed using an alternative technique, eg. ELISA or by reference laboratory.

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