小鼠和人神经前体细胞的椎管内移植

Q2 Biochemistry, Genetics and Molecular Biology Current Protocols in Stem Cell Biology Pub Date : 2018-02-16 DOI:10.1002/9780470151808.sc02d16s26

Jason G. Weinger, Lu Chen, Ronald Coleman, Ronika Leang, Warren C. Plaisted, Jeanne F. Loring, Thomas E. Lane

{"title":"小鼠和人神经前体细胞的椎管内移植","authors":"Jason G. Weinger, Lu Chen, Ronald Coleman, Ronika Leang, Warren C. Plaisted, Jeanne F. Loring, Thomas E. Lane","doi":"10.1002/9780470151808.sc02d16s26","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>This unit describes the preparation and transplantation of human neural precursor cells (hNPCs) and mouse neural precursor cells (mNPCs) into the thoracic region of the mouse spinal cord. The techniques in this unit also describe how to prepare the mouse for surgery by performing a laminectomy to expose the spinal cord for transplantation. NPCs genetically labeled with eGFP transplanted into the spinal cord of a mouse following viral-mediated demyelination can efficiently be detected via eGFP expression. Transplantation of these cells into the spinal cord is an efficacious way to determine their effects in neurological disorders such as multiple sclerosis, Alzheimer's disease, and spinal cord injury. <i>Curr. Protoc. Stem Cell Biol</i>. 26:2D.16.1-2D.16.16. © 2013 by John Wiley & Sons, Inc.</p>\n </div>","PeriodicalId":53703,"journal":{"name":"Current Protocols in Stem Cell Biology","volume":"26 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2018-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/9780470151808.sc02d16s26","citationCount":"5","resultStr":"{\"title\":\"Intraspinal Transplantation of Mouse and Human Neural Precursor Cells\",\"authors\":\"Jason G. Weinger, Lu Chen, Ronald Coleman, Ronika Leang, Warren C. Plaisted, Jeanne F. Loring, Thomas E. Lane\",\"doi\":\"10.1002/9780470151808.sc02d16s26\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <p>This unit describes the preparation and transplantation of human neural precursor cells (hNPCs) and mouse neural precursor cells (mNPCs) into the thoracic region of the mouse spinal cord. The techniques in this unit also describe how to prepare the mouse for surgery by performing a laminectomy to expose the spinal cord for transplantation. NPCs genetically labeled with eGFP transplanted into the spinal cord of a mouse following viral-mediated demyelination can efficiently be detected via eGFP expression. Transplantation of these cells into the spinal cord is an efficacious way to determine their effects in neurological disorders such as multiple sclerosis, Alzheimer's disease, and spinal cord injury. <i>Curr. Protoc. Stem Cell Biol</i>. 26:2D.16.1-2D.16.16. © 2013 by John Wiley & Sons, Inc.</p>\\n </div>\",\"PeriodicalId\":53703,\"journal\":{\"name\":\"Current Protocols in Stem Cell Biology\",\"volume\":\"26 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-02-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1002/9780470151808.sc02d16s26\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current Protocols in Stem Cell Biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/9780470151808.sc02d16s26\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols in Stem Cell Biology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/9780470151808.sc02d16s26","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}

引用次数: 5

摘要

本单元描述了人神经前体细胞(hNPCs)和小鼠神经前体细胞(mNPCs)在小鼠脊髓胸椎区的制备和移植。本单元的技术还描述了如何通过椎板切除术暴露用于移植的脊髓来为手术小鼠做准备。基因标记有eGFP的npc在病毒介导脱髓鞘后移植到小鼠脊髓中，可以通过eGFP表达有效地检测。将这些细胞移植到脊髓中是一种有效的方法，可以确定它们对多发性硬化症、阿尔茨海默病和脊髓损伤等神经系统疾病的影响。咕咕叫。Protoc。干细胞生物学。26:2 .16.1-2 .16.16。©2013 by John Wiley &儿子,Inc。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

Intraspinal Transplantation of Mouse and Human Neural Precursor Cells

This unit describes the preparation and transplantation of human neural precursor cells (hNPCs) and mouse neural precursor cells (mNPCs) into the thoracic region of the mouse spinal cord. The techniques in this unit also describe how to prepare the mouse for surgery by performing a laminectomy to expose the spinal cord for transplantation. NPCs genetically labeled with eGFP transplanted into the spinal cord of a mouse following viral-mediated demyelination can efficiently be detected via eGFP expression. Transplantation of these cells into the spinal cord is an efficacious way to determine their effects in neurological disorders such as multiple sclerosis, Alzheimer's disease, and spinal cord injury. Curr. Protoc. Stem Cell Biol. 26:2D.16.1-2D.16.16. © 2013 by John Wiley & Sons, Inc.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Current Protocols in Stem Cell Biology Biochemistry, Genetics and Molecular Biology-Cell Biology

自引率

0.00%

发文量

期刊介绍： Published in affiliation with the International Society for Stem Cell Research (ISSCR), Current Protocols in Stem Cell Biology (CPSC) covers the most fundamental protocols and methods in the rapidly growing field of stem cell biology. Updated monthly, CPSC will constantly evolve with thelatest developments and breakthroughs in the field. Drawing on the expertise of leading researchers from around the world, Current Protocols in Stem Cell Biology includes methods and insights that will enhance the progress of global research.