利用SHAPE分析病毒RNA结构

Current Protocols in Microbiology Pub Date : 2018-02-16 DOI:10.1002/9780471729259.mc15h03s30

Cecily P. Burrill, Raul Andino

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引用次数: 3

摘要

通过引物延伸(SHAPE)分析选择性2’羟基酰化为研究RNA结构提供了一种比传统方法具有更高分辨率和更高通量的手段。我们提出了几种方案，这些方案基于各种先前发表的方法，并针对Andino实验室的脊髓灰质炎病毒RNA分析进行了调整和优化。这些方法包括非变性RNA提取、RNA修饰和引物扩展以及ShapeFinder中的数据处理。咕咕叫。Protoc。Microbiol 30:15H.3.1-15H.3.12。©2013 by John Wiley &儿子,Inc。

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RNA Structure Analysis of Viruses Using SHAPE

Selective 2′ hydroxyl acylation analyzed by primer extension (SHAPE) provides a means to investigate RNA structure with better resolution and higher throughput than has been possible with traditional methods. We present several protocols, which are based on a variety of previously published methods and were adapted and optimized for the analysis of poliovirus RNA in the Andino laboratory. These include methods for nondenaturing RNA extraction, RNA modification and primer extension, and data processing in ShapeFinder. Curr. Protoc. Microbiol. 30:15H.3.1-15H.3.12. © 2013 by John Wiley & Sons, Inc.

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Current Protocols in Microbiology Immunology and Microbiology-Parasitology

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期刊介绍： Current Protocols in Microbiology provides detailed, step-by-step instructions for analyzing bacteria, animal and plant viruses, fungi, protozoans and other microbes. It offers updated coverage of emerging technologies and concepts, such as biofilms, quorum sensing and quantitative PCR, as well as proteomic and genomic methods. It is the first comprehensive source of high-quality microbiology protocols that reflects and incorporates the new mandates and capabilities of this robust and rapidly evolving discipline.

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